Part of Thermo Fisher Scientific
Items which require this product:
Organisms this product works with:
Code: CN0020
Description
CampyGen Compact for 1 or 2 Petri dishes, is a simple system for generating
microaerobic conditions. The system consists of a plastic pouch and sealing
clip and a paper gas generating sachet. The paper sachet contains ascorbic
acid which reacts on contact with air to produce the microaerobic conditions
for
the growth of microaerophilic organisms.
Components
20 CampyGen Compact paper sachets, individually wrapped in foil
1 product leaflet
Materials Required but not Provided
Sealing Clips (AN0005)
Plastic Pouches (AG0020)
W-Zip Plastic Pouches (AG0060)
Precautions
This product is for in vitro use only. The CampyGen Compact paper
sachet will become active on contact with air. It is essential that the plastic
pouch is sealed within one minute of exposing the paper sachet to the air. The
reaction of ascorbic acid with oxygen is exothermic. However, the temperature
of the CampyGen Compact paper sachet will not exceed 65°C.
Storage
Store at 2-25°C. Under these conditions, the CampyGen Compact
sachets will retain their activity until the expiry date given on the outer
box and on the foil wrap of the sachets.
Directions
1 Place 2 inoculated plates in a plastic pouch. Disposable plastic
Petri dishes should be of the vented variety to aid gas transfer between the
interior
and exterior of the plates. If only one plate is to be inoculated, an uninoculated
plate should also be placed in the plastic pouch to prevent further activity
as the volume of O2 and CO2 is critical.
2 Tear open a CampyGen Compact foil sachet at the tear-nick indicated.
Remove the CampyGen Compact paper sachet from within.
3 Immediately place the paper sachet in the plastic pouch with the plates.
N.B. The paper sachet will become warm to the touch on exposure to air.
4 Expel excess air from the plastic pouch. Seal the plastic
pouch immediately with a sealing clip or w-zip closure as appropriate. The
time taken between opening the foil sachet and sealing
the plastic pouch should not exceed 1 minute.
5 Incubate appropriately.
6 After the incubation period, remove the plates and examine
for the presence of colonies. If the plates require re- incubation, a fresh
CampyGen
Compact sachet must be used, following steps 2-5 described above. N.B.
The plates may be initially inspected through the transparent plastic pouch.
If the bag is opened, a fresh CampyGen Compact sachet is required for re-incubation.
7 After incubation, the exhausted CampyGen Compact paper sachet and plastic
pouch should be sterilised and discarded with the non-hazardous laboratory waste.
Control Testing
The user should check their technique periodically for the ability
to provide adequate conditions for the growth of microaerophilic bacteria.
Campylobacter
jejuni ATCC® 33291 may be used for this purpose.
Disposal
On removal from the pouch after incubation, the CampyGen Compact paper sachet
will retain a small amount of activity and become warm. The sachets should be
allowed to cool to room temperature prior to sterilisation and disposal with
the non-hazardous laboratory waste.