Part of Thermo Fisher Scientific
Items which require this product:
Code: AN0010 & AN0020
AnaeroGen Compact is a simple system for the anaerobic incubation of up to 4 Petri dishes or an identification panel.
Description
The system consists of a plastic pouch and a paper gas generating sachet. The paper sachet contains ascorbic acid and activated carbon which reacts on with air. Oxygen is rapidly absorbed and carbon dioxide is produced. When the paper sachet is placed in a sealed plastic pouch, this reaction will create ideal atmospheric conditions for the growth of anaerobes. It proceeds with no evolution of hydrogen, and therefore does not require a catalyst. No water is required to activate the reaction. This gives the system many advantages over the commonly used borohydride systems including increased safety and convenience.
When used as directed, the AnaeroGen Compact sachet will reduce the oxygen content in the pouch to below 1% within 30 minutes. The resulting carbon dioxide content will be between 8% and 14%. The level of carbon dioxide will depend on how many plates are placed in the pouch. AnaeroGen Compact has been designed for use with 1-4 plates.
Two styles of pouch are available. The first requires the use of a plastic clip (AN0005) to make a sufficient seal to ensure anaerobic conditions are established and maintained. The second, the W-Zip pouch, is a more convneient product to use, as it has an intergral seal, which removes the need for the clip. The W-Zip pouch is easy to close; pinch the seal together at one end, then run the fingers along, squeezing all the way across, to ensure there are no gaps. An airtight seal is thus, generated.
The active components within each AnaeroGen Compact sachet are ascorbic acid and activated carbon.
COMPONENTS
Each box contains:
Product Components Table | AN0010C | AN0020C | AN0010W |
Anaerogen Compact Sachets | 10 | 20 | 10 |
Plastic Pouches | 10 | x | x |
W-Zip Plastic Pouches | x | x | 10 |
Product Insert | 1 | 1 | 1 |
MATERIALS REQUIRED BUT NOT PROVIDED
Additional materials required | AN0010C | AN0020C | AN0010W | |
either | or | |||
Anaerogen Compact Sealing Clips - 5 clips per pack (AN0005C) | yes | yes | ||
Plastic Pouches - 20 pouches per pack (AG0020C) | yes | |||
W-Zip Plastic Pouches - 20 pouches per pack (AG0060C) | yes |
Precautions
This product is for in vitro use only.
The AnaeroGen Compact paper sachet will become active on contact with air. It is therefore essential that the paper sachet is placed into the pouch and the pouch sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the AnaeroGen Compact paper sachet will not exceed 65°C.
Once the oxygen in the pouch has been absorbed, the temperature within the pouch will return to ambient temperature.
Storage
Store at 2-25°C. Under these conditions, the AnaeroGen Compact sachets will retain their activity until the expiry date given on the outer box and on the foil wrapped sachet.
Directions
Control Testing
It is recommended that OXOID Anaerobic Indicator (BR0055) is also used in the plastic pouch as a visual check that anaerobic conditions have been achieved and maintained.
The user should check their anaerobic technique periodically for its ability to provide adequate conditions for the growth of anaerobic bacteria.
The following strains are recommended:
Positive control: | Expected reactions |
Clostridium novyii ATCC® 9690 | Growth |
Negative control: | |
Kocuria rhizophila ATCC® 9341* | No growth |
Disposal
On removal from the pouch after incubation, the AnaeroGen Compact paper sachet will retain a small amount of activity and become warm. The sachets should be allowed to cool to room temperature prior to sterilisation and disposal with the non-hazardous laboratory waste.