Part of Thermo Fisher Scientific
Organisms this product works with:
Other products used in the isolation of Clostridium difficile :
Code: AN0025 & AN0035
Description
Where an AnaeroGen sachet is placed in a sealed jar, the atmospheric oxygen
in the jar is rapidly absorbed with the simultaneous generation of carbon dioxide.
This novel method differs from others commonly used in that the reaction proceeds
with no evolution of hydrogen, and therefore, does not require a catalyst.
Furthermore, water is not required to activate the reaction.
When used as directed, the AnaeroGen sachet will reduce the oxygen level in
the jar to below 1% within 30 minutes. The resulting carbon dioxide level will
be between 9% and 13%.
AnaeroGen was used in methodology for detecting bifidobacteria in meat and meat
products in an investigation into the suitability of these organisms as indicators
of faecal contamination.
Components
Each box contains:
10 AnaeroGen paper sachets which are individually foil packed.
1 Product Insert.
The active component within each AnaeroGen sachet is ascorbic acid.
Precautions
This product is for in-vitro use only.
As soon as the AnaeroGen paper sachet is exposed to the air, the reaction will
start. It is therefore essential that the paper sachet is placed in the jar
and the jar sealed within one minute.
The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature
of the AnaeroGen paper sachet will not exceed 65°C.
Storage
Store at 2-25°C. Under these conditions, the AnaeroGen sachets will
retain their reactivity until the expiry date given on the outer box and
on the foil sachet.
Directions
AN0035 is designed for use in 3.5 litre jars. It is also suitable for
use in the Oxoid Anaerobic Jar HP0011 and for other jars of similar capacity.
AN0025 is designed for use in 2.5 litre jars such as the new Oxoid AnaeroJar AG0025
and other jars of similar capacity.
1 Place the inoculated media plates in the appropriate anaerobic
jar. Disposable plastic Petri dishes should be of the vented variety to aid
gas transfer
between the interior and exterior of the plates.
2 Tear open an AnaeroGen foil sachet at the tear-nick indicated, and
remove the AnaeroGen paper sachet from within.
3 Immediately place the AnaeroGen paper sachet in the appropriate clip
on the plate carrier within the jar.
N.B. The AnaeroGen paper sachet will become warm to touch on exposure
to air.
4 Close the jar lid immediately.
N.B. The time taken between opening the foil sachet and sealing the jar
should not exceed 1 minute. Extended exposure will result in loss of reactivity,
and full anaerobic conditions may not be achieved in the jar.
5 After the appropriate incubation period remove the plates and examine
for the presence of anaerobes. If the plates require re-incubation then a fresh
AnaeroGen sachet must be used following steps
2-5 described above.
6 After incubation, the exhausted AnaeroGen sachet should be discarded
with the appropriate laboratory waste.
Control Testing
It is recommended that an OXOID Anaerobic Indicator (BR0055) is used
in the jar as a visual check that anaerobic conditions have been achieved and
maintained.
The user should check their Anaerobic system periodically for its ability to
provide adequate conditions for the growth of appropriate bacteria.
The following strains are recommended:
Clostridium novyii ATCC® 9690 growth
Kocuria rhizophila ATCC® 9341 no growth
Disposal
On removal from the jar after incubation, the AnaeroGen paper sachet will
retain a small amount of reactivity and will warm up. The sachets should
be allowed to cool at room temperature prior to disposal alongside the appropriate
laboratory waste.
Reference
1 Beerens H. (1998) Int. J. Food Microbiol. 40. 203-207.