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CO₂GEN COMPACT

Code: CD0020

Description
CO₂Gen Compact is a simple system for the generation of a carbon dioxide-enriched atmosphere for the incubation of 2 Petri dishes. The system consists of a plastic pouch and sealing clip (alternatively a w-zip pouch can be used) and a paper gas generating sachet. The paper sachet contains ascorbic acid which reacts with air to produce an atmosphere which contains approximately 6% carbon dioxide. The final concentration of oxygen is 15%.

Components
20 CO₂Gen Compact paper sachets, individually wrapped in foil
1 product leaflet

Materials Required but not Provided
Sealing Clips (AN0005)
Plastic Pouches (AG0020) or
W-Zip Pouches (AG0060)

Precautions
This product is for in vitro use only. The CO₂Gen Compact paper sachet will become active on contact with air. It is therefore essential that the paper sachet is placed in the pouch and the pouch sealed within one minute. The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the CO₂Gen Compact paper sachet will not exceed 65°C.

Storage
Store at 2-25°C. Under these conditions, the CO₂Gen Compact sachets will retain their activity until the expiry date given on the outer box and on the foil wrap of the sachets.

Directions
1 Place 2 inoculated media plates in a plastic pouch. Disposable plastic Petri dishes should be of the vented variety to aid gas transfer between the interior and exterior of the plates. If only one plate is to be inoculated, an uninoculated plate should also be placed in the plastic pouch.
2 Tear open a CO₂Gen Compact foil sachet at the tear-nick indicated. Remove the CO₂Gen Compact paper sachet from within.
3 Immediately place the paper sachet in the plastic pouch. N.B. The paper sachet will become warm to the touch on exposure to air.
4 Expel excess air from the plastic pouch. Seal the plastic pouch immediately with a sealing clip or w-zip closure as appropriate. The time taken between opening the foil sachet and sealing the plastic pouch should not exceed 1 minute.
5 Incubate appropriately.
6 After the incubation period, remove the plates and examine for the presence of colonies. If the plates require re- incubation, a fresh CO₂Gen Compact sachet must be used following steps 2-5 described above. N.B. The plates may be initially inspected through the transparent plastic pouch. If the bag is opened, a fresh CO₂Gen Compact sachet is required for re-incubation.
7 After incubation, the exhausted CO₂Gen Compact paper sachet and plastic pouch should be sterilised and discarded with the non-hazardous laboratory waste.

Disposal
On removal from the pouch after incubation, the CO₂Gen Compact paper sachet will retain a small amount of activity and become warm. The sachets should be allowed to cool to room temperature prior to sterilisation and disposal with the non- hazardous laboratory waste.