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Dehydrated Culture Media

NUTRIENT AGAR

Code: CM0003 (Powder)
Code: CM0004 (Tablets)

A general purpose medium which may be enriched with up to 10% blood or other biological fluid.

Typical Formula *

gm/litre

`Lab-Lemco’ powder

1.0

Yeast extract

2.0

Peptone

5.0

Sodium chloride

5.0

Agar

15.0

pH 7.4 ± 0.2 @ 25°C

 
* Adjusted as required to meet performance standards.

Directions
Powder: Suspend 28g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Tablets: Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Nutrient Agar is a basic culture medium used to subculture organisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.

In semi-solid form, agar slopes or agar butts, the medium is used to maintain control organisms1.

Nutrient Agar is suitable for teaching and demonstration purposes. It contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid, as required. The medium, without additions, may be used for the cultivation of organisms which are not exacting in their nutritional requirements.

For a medium which is richer in nutrients, see Blood Agar Base No.2 CM0271.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel

Quality control

Positive controls:

Expected results

Staphylococcus aureus ATCC® 25923 *

Good growth; straw/white colonies

Escherichia coli ATCC® 25922 *

Good growth; straw colonies

Negative control:

 

Uninoculated medium

No change

* This organism is available as a Culti-Loop®

Reference
1. Lapage S. P., Shelton J. E. and Mitchell T. G. (1970)` in Methods in Microbiology’ Eds. Norris J. R. and Ribbons D. W. Vol.3A. Academic Press. London. p.116.

 
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