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Dehydrated Culture Media

`LAB-LEMCO’ BROTH

Code: CM0015

A nutrient broth for general bacteriological use, and for the examination of water, sewage, and dairy products by American standard methods.

Typical Formula*

gm/litre

`Lab-Lemco’ powder

3.0

Peptone

5.0

pH 7.4 ± 0.2

* Adjusted as required to meet performance standards

Directions
Dissolve 8g in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Description
`Lab-Lemco’ Broth is a general purpose liquid medium used for the examination of water, dairy and food products1,2,3.
It is used as a nutrient meat extract broth for general bacteriology.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.
`Lab-Lemco’ Broth is slightly more alkaline than the medium used in the APHA publications (pH 6.8).

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured solution

Quality control

Positive controls:

Expected results

Staphylococcus aureus ATCC® 25923*

Turbid growth.

Escherichia coli ATCC® 25922 *Turbid growth.

Negative control:

 

Uninoculated medium.

No change

* This organism is available as a Culti-Loop®

References
1. American Public Health Association (1980) Standard Methods for the Examination of Water and Wastewater. 15th Edn. APHA Inc. Washington DC.
2. American Public Health Association (1978) Standard Methods for the Examination of Dairy Products. 14th Edn. APHA Inc. Washington DC.
3. American Public Health Association (2001) Compendium of Methods for the Microbiological Examination of Foods 4th Edn. .

 
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