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Material Safety Data Sheet

Organisms

Organisms in the Clinical sector this product works with:

Dehydrated Culture Media

Sector: Clinical

THIOGLYCOLLATE MEDIUM (BREWER)

Code: CM0023

An anaerobic medium especially useful for the sterility control of solutions containing mercury preservatives.

Typical Formula*

gm/litre

`Lab-Lemco’ powder

1.0

Yeast extract

2.0

Peptone

5.0

Glucose

5.0

Sodium chloride

5.0

Sodium thioglycollate

1.1

Methylene blue

0.002

Agar

1.0

pH 7.2 ± 0.2

 
* Adjusted as required to meet performance standards

Directions
Suspend 20g in 1 litre of distilled water. Bring to the boil, mix well and allow to stand until completely dissolved. Distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Media containing small quantities of agar are liable to separate if cooled rapidly. Tubes of reconstituted and autoclaved medium should be allowed to cool slowly on a wooden surface in a draught-free atmosphere.

Description
This anaerobic medium, developed by Brewer1,2,3 is used principally for testing the sterility of biological products.

The medium contains a small concentration of methylene blue as an oxidation-reduction indicator. If more than 20% of the uppermost portion of the stored medium has changed to a green colour, anaerobic conditions may be restored by heating in a boiling water bath or steamer for 5 to 10 minutes. This treatment must not be repeated.

Thioglycollate Medium (Brewer) is especially useful for the control of biological solutions containing mercurial preservatives, the toxicity of the latter being neutralised by the thioglycollate.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at room temperature.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw/yellow coloured solution with green layer

Quality control

Positive controls:

Expected results

Bacterioides vulgatus ATCC® 8482

Turbid growth; and/or single colonies

Clostridium sporogenes ATCC® 19404 *

Turbid growth; and/or single colonies

Negative control:

 

Uninoculated medium

No change

* This organism is available as a Culti-Loop®

Precautions
Check the upper portion of the prepared medium before inoculation. If more than one-third of the fluid is oxidised i.e. green coloured, discard the bottle.

If one-third or less fluid is oxidised, then heat in boiling water with the cap loosened to drive off oxygen. Cool to room temperature before inoculation. This reheating process can only be carried out once because of the formation of toxic radicles in the medium.

Organisms which ferment glucose and lower the pH to critical levels may not survive in this medium after growth has taken place.

References
1. Brewer J. H. (1940) JAMA. 115. 598-600.
2. Brewer J. H. (1940) J. Bact. 39. 10-13.
3. Brewer J. H. (1943) J. Bact. 46. 395-398.

 
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