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Material Safety Data Sheet

Organisms

Organisms this product works with:

Dehydrated Culture Media

SALT MEAT BROTH

Code: CM0094 (Tablets)

An enrichment broth for halophilic organisms, especially staphylococci.

Typical Formula*

gm/litre

Peptone

10.0

`Lab-Lemco’ powder

10.0

Neutral ox-heart tissue

30.0

Sodium chloride

100.0

pH 7.6 approx. @ 25°C

 
* Adjusted as required to meet performance standards

Directions
Add 2 tablets to 10ml of distilled water in an appropriately sized test tube and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Salt Meat Broth is an enrichment medium for the isolation of staphylococci from grossly contaminated specimens such as faeces, particularly during the investigation of staphylococcal food poisoning. Salt meat medium will detect small numbers of staphylococci when mixed with large numbers of other bacteria1,2.

The medium is also an excellent substrate for the cultivation of some of the halophilic micrococci associated with hides and raw salt supplies. It should be noted that staphylococci growing in this medium cannot be directly tested for coagulase production - they should first be sub-cultured on a medium which contains less salt. Blood Agar Base CM0055 is recommended for this purpose.

Technique
For the isolation of Staphylococcus aureus from samples of food, emulsify the specimen in Peptone Water CM0009, and inoculate a tube of Salt Meat Broth. After 24-48 hours incubation at 35°C, discrete colonies may be obtained by plating out a small portion of the culture onto Mannitol Salt Agar CM0085 or Staphylococcus Medium No.110 CM0145.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Appearance
Tablets: Straw coloured, with brown granules
Prepared medium: Straw coloured solution, with brown granules

Quality control

Positive control:

Expected results

Staphylococcus aureus ATCC® 25923 *

Good growth.

Negative control:

 

Uninoculated medium.

No change

* This organism is available as a Culti-Loop®

References
1. Maitland H. B. and Martyn G. (1948) J. Path. Bact. 60. 553-557.
2. Fairbrother R. W. and Southall J. E. (1950) Mon. Bull. Min. Hlth Pub. Hlth Serv. 9. 170-172.

 
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