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Material Safety Data Sheet

Organisms

Organisms this product works with:

Dehydrated Culture Media

CZAPEK DOX LIQUID MEDIUM (MODIFIED)

Code: CM0095

A defined fluid medium for the cultivation of those fungi and bacteria which are able to utilise sodium nitrate as the sole source of nitrogen.

Typical Formula*

gm/litre

 Sodium nitrate

2.0

 Potassium chloride

0.5

 Magnesium glycerophosphate

0.5

 Ferrous sulphate

0.01

 Potassium sulphate

0.35

 Sucrose

30.0

 pH 6.8 ± 0.2 @ 25°C

 

* Adjusted as required to meet performance standards

Directions
Add 33.4g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Description
A defined fluid medium for the cultivation of fungi and bacteria capable of utilising sodium nitrate as the sole source of nitrogen.

Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.
Store tubes of broth at 15-25°C.

Appearance
Dehydrated medium: White coloured, free-flowing powder
Prepared medium: Colourless solution

Quality control

Positive controls:

Expected results

Aspergillus niger ATCC® 9642 *

Good growth; white mycelium with black spores

Candida albicans ATCC® 10231 *

Suspended growth

Negative control:

 

Uninoculated medium

No change

* This organism is available as a Culti-Loop®

References
1. Dawson Christine O. (1962) Saboutaudia 1. 214-219.
2. Smith G. (1960) `An Introduction to Industrial Mycology’ 5th ed., Edward Arnold Ltd., London.
3. Thom C. and Church M. B. (1926) `The Aspergilli’ Williams and Wilkins Co., Baltimore.
4. Thom C. (1930) `The penicillia’ Williams and Wilkins Co., Baltimore.
5. Raper K. B. and Thom C. (1949) `Manual of the Penicillia’ Williams and Wilkins Co., Baltimore.
6. Wakesman S. A. (1931) `Principles of soil Microbiology’ Bailliere Tindall and Cox, London.
7. Brooks F. T. and Kidd M. N. (1921) Specia. Report No.6, Food Invest. Board, DSIR, London.
8. Brooks F. T. and Handsford C. G. (1922) Trans. Brit. Mycol. Soc. 8. 113-142.

 
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