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Material Safety Data Sheet

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Organisms

Organisms this product works with:

Dehydrated Culture Media

TOMATO JUICE AGAR

Code: CM0113

A medium for the cultivation and enumeration of Lactobacillus species.

Typical Formula*

gm/litre

Tomato juice (solids from 400ml)

20.0

Peptone

10.0

Peptonised milk

10.0

Agar

12.0

pH 6.1 ± 0.2 @ 25°C

* Adjusted as required to meet performance standards

Directions
Suspend 52g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.

Description
Tomato Juice Agar is recommended for the cultivation and enumeration of Lactobacillus species. This is a modification of Kulp’s medium for the culture of lactobacilli, its preparation differing only slightly from the method described by Kulp & White1.

When a more acid medium is required, for the direct plate count of lactobacilli and other organisms from saliva, the pH of the medium may be adjusted to approximately 5.1 by the addition of 1ml of 10% Lactic Acid SR0021 to each 100ml of sterilised medium.

Davis2 compared the morphology of Lactobacillus colonies on Oxoid Tomato Juice Agar and other media.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the plates of prepared medium at 2-8°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Dark straw coloured gel

Quality control

Positive control:

Expected results

Lactobacillus fermentum ATCC® 9338

Good growth, cream colonies

Negative control:

 

Uninoculated medium

No change

References
1. Kulp J. W. L. and White V. (1932) Science 76. 17-18.
2. Davis G. H. G. (1959) Lab. Prac. 8(5). 161-167.

 
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