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Dehydrated Culture Media

ANTIBIOTIC MEDIUM NO. 3 ASSAY BROTH

Code: CM0287

used in the serial dilution assay of penicillin and other antibiotics

Typical Formula*

gm/litre

Peptone

5.0

Yeast extract

1.5

`Lab-Lemco’ powder

1.5

Glucose

1.0

Sodium chloride

3.5

Dipotassium hydrogen phosphate

3.68

Potassium dihydrogen phosphate

1.32

pH 7.0 ± 0.2 @ 25°C

 
 * Adjusted as required to meet performance standards

Directions
Add 17.5g to 1 litre of warm (60°C) distilled water and mix well to dissolve. Distribute and sterilise by autoclaving at 121°C for 15 minutes.

Description
Medium No.3 is used in the turbidimetric assay of penicillin and tetracycline with Staphylococcus aureus.

Taking advantage of modern technology, this medium is based on the original `Penassay Broth’ which has been withdrawn.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured solution

Quality control

Positive control:

Expected results

Staphylococcus aureus ATCC® 25923 * Turbid growth
Negative control:  
Uninoculated mediumNo change
* This organism is available as a Culti-Loop®
 
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