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Material Safety Data Sheet

Organisms

Organisms this product works with:

Dehydrated Culture Media

MRS BROTH (DE MAN, ROGOSA, SHARPE)

Code: CM0359

A non-selective medium for profuse growth of `lactic acid bacteria’.

Typical Formula*

gm/litre

Peptone

10.0

`Lab-Lemco’ powder

8.0

Yeast extract

4.0

Glucose

20.0

Sorbitan mono-oleate

1 ml

Dipotassium hydrogen phosphate

2.0

Sodium acetate 3H2O

5.0

Triammonium citrate

2.0

Magnesium sulphate 7H2O

0.2

Manganese sulphate 4H2O

0.05

pH 6.2 ± 0.2 @ 25°C

 

* Adjusted as required to meet performance standards 

Directions
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Description
MRS Broth may be used for tests in the identification of lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith1.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated medium: Dark straw powder
Prepared medium: Amber coloured solution

Quality control

Positive control:

Expected results

Lactobacillus gasseri ATCC® 19992 *

Turbid growth

Negative control:

 

Uninoculated medium

No change

* This organism is available as a Culti-Loop®

Reference
1. Sharpe M. Elisabeth, Fryer T. F. and Smith D. G. (1966) `Identification of the Lactic Acid Bacteria’ in `Identification Method for Microbiologists Part A’ (Gibbs B. M. and Skinner F. A. eds.) London and New York, Academic Press. Pages 65-79.

 
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