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Dehydrated Culture Media

Sector: Clinical

ISO-SENSITEST BROTH

Code: CM0473

a semi defined medium designed for antimicrobial susceptibility testing

Typical Formula*

gm/litre

Hydrolysed casein

11.0

Peptones

3.0

Glucose

2.0

Sodium chloride

3.0

Soluble starch

1.0

Disodium hydrogen phosphate

2.0

Sodium acetate

1.0

Magnesium glycerophosphate

0.2

Calcium gluconate

0.1

Cobaltous sulphate

0.001

Cupric sulphate

0.001

Zinc sulphate

0.001

Ferrous sulphate

0.001

Manganous chloride

0.002

Menadione

0.001

Cyanocobalamin

0.001

L-Cysteine hydrochloride

0.02

L-Tryptophan

0.02

Pyridoxine

0.003

Pantothenate

0.003

Nicotinamide

0.003

Biotin

0.0003

Thiamine

0.00004

Adenine

0.01

Guanine

0.01

Xanthine

0.01

Uracil

0.01

pH 7.4 ± 0.2  @ 25°C

 

*Adjusted as required to meet performance standards

Directions
Add 23.4g to 1 litre of distilled water. Mix well and heat to dissolve. Distribute into tubes or bottles. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Oxoid Iso-Sensitest™ Broth has been produced in parallel with Iso-Sensitest Agar CM0471. The broth has an identical nutrient formulation without the specially purified agar. Where studies on antimicrobial susceptibilities are to be made in both broth and agar, this will be found to be of particular value. Details of the function of the medium and the methodology used for antimicrobial susceptibility tests are discussed in the Section, Susceptibility Testing.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Pale straw coloured solution

Quality Control

Positive controls:

Expected results

Streptococcus pneumoniae ATCC® 49619 *

Turbid growth
(with blood) 
Staphylococcus aureus ATCC® 25923 *Turbid growth
Negative control:  
Uninoculated mediumNo change
* This organism is available as a Culti-Loop®

References
1.
Ericsson H. M. and Sherris J. C. (1971) Acta. Pathol. Microbiol. Scand. Suppl. 217. 1-90.
2. Garrod L. P. and Waterworth P. M. (1971) J. Clin. Path. 24. 779-789.
3. Reller L. B., Schoenknecht F. D., Kenny M. A. and Sherris J. C. (1974) J. Infect. Dis. 130. 454-463.
4. Duncan I. B. R. (1974) Antimicrob. Agents & Chemotherapy 5. 9-15.
5. Yourassowsky E., Vanderlinden M. P. and Schoutens E. (1974) J. Clin. Path. 27. 897-901.
6. Neussil H. (1976) Chemotherapy Vol.2. 33-40.
7. Bridson E. Y. (1976) Arztl. Lab. 22. 373-376.
8. Tanner E. I. and Bullin C. H. (1974) J. Clin. Path. 27. 565-568.
9. Thomas M. and Bond L. (1973) Med. Lab. Technol. 30. 277-279.
10. Barker J., Healing D. and Hutchinson J. G. P. (1972) J. Clin. Path. 25. 1086-1088.
11. Reynolds A. V., Hamilton-Miller J. M. T. and Brumfitt W. (1974) B.M.J. (ii) 778.
12. Stewart Sheila M., Anderson Isobel M. E. and Malcolm Margaret G. G. (1975) J. Clin. Path. 28. 195-197.
13. Bell S. M. (1975) Pathology 7. Suppl. 1-48.
14. Garrod L. P. and Waterworth P. M. (1969) J. Clin. Path. 22. 534-538.
15. Gilbert D. N., Kutscher E., Ireland P., Barnett J. A. and Sandford J. P. (1971) J. Infect. Dis. 124. (suppl.), S37-S45.
16. Zimelis V. M. and Jackson G. G. (1973) J. Infect. Dis. 127. 663-669.
17. Davis S. D., Ianetta A., Wedgewood R. J. (1971) J. Infect. Dis. 124. 610-612.
18. Brenner V. C. and Sherris J. C. (1972) Antimicrob. Agents Chemother. 1. 116-122.
19. Traub W. H. (1970) Appl. Microbiol. 20. 98-102.
20. Acar J. F. (1980) Antibiotics in Laboratory Medicine, Lorian V. (Ed.) Williams and Wilkens, Baltimore, USA, 48-51.
21. Hartzen S.H., Andersen L.P., Bremmelgaard A. et al (1997) Antimicrob. Ag. and Chemother. 41. 2634-2639.
22. British Society for Antimicrobial Chemotherapy (2002) Disc Diffusion Method for Antimicrobial Susceptibility Testing. Version 2.1.1. BSAC.

ATCC® is a registered trademark of American Type Culture Collection

 
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