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Dehydrated Culture Media

SCHAEDLER ANAEROBE BROTH

Code: CM0497

A broth version of Schaedler Anaerobe Agar CM0437 for the general growth of anaerobes and for use in blood cultures and antibiotic MIC studies of these organisms.

Typical Formula*

gm/litre

Tryptone Soya Broth (Oxoid CM129)

10.0

Special peptone

5.0

Yeast extract

5.0

Glucose

5.0

Cysteine HCl

0.4

Haemin

0.01

Tris Buffer

0.75

pH 7.6 ± 0.2 @ 25°C

 
* Adjusted as required to meet performance standards

Directions
Add 26.5g to 1 litre of distilled water and mix to dissolve completely. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Description
Schaedler Anaerobe Broth is a clear medium which can support the growth of those anaerobic bacteria commonly associated with human and veterinary disease. It is identical to the formula of Schaedler Anaerobe Agar CM0437, except that the agar has been omitted.

Used as a fluid medium, under the appropriate atmosphere, Schaedler Anaerobe Broth showed enhanced growth with a number of demanding anaerobic organisms when compared with seven other commonly used broth media1.

Schaedler Anaerobe Broth can also be used to determine antibiotic MIC levels of anaerobic organisms. The extreme variations in growth rates usually prevent the existing linear regression plots of MIC versus zone diameter being used. The use of tube methods overcomes this problem1.
Fass, Prior and Rotilie2 described a simple tube method that does not require special atmospheres or special equipment to carry out the test. By adding a 6mm solid glass bead to the tube of broth prior to autoclaving, growth of most organisms could be detected after incubation for one day, by slowly rotating the tube and observing the swirl of organisms. The addition of 0.0001 of w/v resazurin to the medium was used to determine whether oxidation had occurred in stored media. For anaerobic cocci, heat-inactivated horse serum was added to a final concentration of 1% v/v before use3.

The addition of menadione (0.1 g/litre), sodium polyanethol-sulphonate (SPS, 0.3 g/litre) and carbon dioxide (3% v/v) to Schaedler Broth enables it to be used as a blood culture medium and for the cultivation of especially fastidious Bacteroides species.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared broth in the dark at <15°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured solution

Quality control

Positive controls:Expected Results
Clostridium perfringens ATCC® 13124Turbid growth
Bacteroides fragilis ATCC® 25285*Turbid growth
Prevotella loescheii ATCC® 15930
(with menadione addition)
Turbid growth
Negative control: 
UninoculatedNo change
* This organism is available as a Culti-loop®

Precautions
As with all anaerobic broth media, it is important to avoid chemo-oxidation (overheating) and photo-oxidation (storage in the light) because such oxidative effects cause the inhibition of growth.

References
1. Stalons D. R., Thornsberry C. and Dowel V. R. (1974) Appl. Microbiol. 27. 1098-1104.
2. Fass R. J., Prior R. B. and Rotilie C. A. (1975) Antimicrob. Agents Chemother. 8. 444-452.
3. Rotilie C. A., Fass R. J., Prior R. B. and Perkins R. L. (1975) Antimicrob. Agents Chemother. 7. 311-315.

 
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