Part of Thermo Fisher Scientific

Thermo Scentific
 
 
 

Material Safety Data Sheet

Optional Products

Organisms

Organisms this product works with:

Dehydrated Culture Media

WL NUTRIENT BROTH

Code: CM0501

A liquid medium for use in the control of brewing and other fermentation processes.

Typical Formula*

gm/litre

Yeast extract

4.0

Tryptone

5.0

Glucose

50.0

Potassium dihydrogen phosphate

0.55

Potassium chloride

0.425

Calcium chloride

0.125

Magnesium sulphate

0.125

Ferric chloride

0.0025

Manganese sulphate

0.0025

Bromocresol green

0.022

pH 5.5 ± 0.2 @ 25°C

 

* Adjusted as required to meet performance standards

Directions
Dissolve 60g in 1 litre of distilled water. Mix well and distribute into containers. Sterilise by autoclaving at 121°C for 15 minutes.
The pH may be raised to 6.5 by the addition of 1% w/v sodium bicarbonate solution. Adding 0.004 grams/litre of cycloheximide per litre (4ml SR0222) will form WL Differential Broth. Note the precautions to be taken under HAZARDS.

Description
WL Nutrient Broth is based on the formulation of Green and Gray1 and is used where there are advantages for broth media e.g. using larger samples of liquid products or for enrichment cultures with cycloheximide.

Addition of 0.004 grams/litre of cycloheximide suppresses yeast growth and renders the medium selective for bacterial contaminants. Adjustment of the medium to pH 6.5 facilitates growth of bakers’ and distillers’ yeasts; the medium at pH 5.5 is used for growth of bakers’ yeasts.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated medium: Pale blue coloured, free-flowing powder
Prepared medium: Blue/green coloured solution

Quality control

Positive controls: w/o cycloheximide

Expected results

Saccharomyces cerevisiae ATCC® 9763 *

Turbid growth

Negative control:

 

Uninoculated medium

No change

Positive control: with cycloheximide  
Escherichia coli ATCC® 25922 *Turbid growth

Negative control:

 
Saccharomyces cerevisiae ATCC® 9763 *No growth
* This organism is available as a Culti-Loop®

Precautions
When handling cycloheximide observe the precautions to be taken under HAZARDS.

References
1. Green S. R. and Gray P. P. (1950) Wallerstein Lab. Comm. 13. 357.
2. Hall Jean F. (1971) J. Inst. Brewing 77. 513-516.

 
©2001 - 2021 Oxoid Limited, All rights reserved.
Copyright, Disclaimer and Privacy Policy | Conditions of Sale | About Us | Cookies
Thermo Fisher Scientific Inc.