Part of Thermo Fisher Scientific
Organisms in the Clinical sector this product works with:
Other products used in the isolation of Campylobacter:
CAMPYLOBACTER SELECTIVE AGAR (PRESTON)
a selective medium which when prepared from Campylobacter Agar Base CM0689, Preston Campylobacter Selective Supplement SR0117 and Lysed Horse Blood SR0048, can be used for the selective isolation of Campylobacter jejuni and C. coli from human, animal, avian and environmental specimens
CAMPYLOBACTER AGAR BASE
Code: CM0689
Typical Formula* | gm/litre |
`Lab-Lemco’ powder | 10.0 |
Peptone | 10.0 |
Sodium chloride | 5.0 |
Agar | 12.0 |
pH 7.5 ± 0.2 @ 25°C |
CAMPYLOBACTER SELECTIVE SUPPLEMENT (PRESTON)
Code: SR0117
Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
Polymyxin B | 2,500IU | 5,000IU |
Rifampicin | 5.0mg | 10.0mg |
Trimethoprim | 5.0mg | 10.0mg |
Cycloheximide | 50.0mg | 100.0mg |
MODIFIED PRESTON CAMPYLOBACTER SELECTIVE SUPPLEMENT
Code: SR0204
Vial contents (each vial is sufficient for 500ml of medium) | per vial | per litre |
Polymyxin B | 2,500IU | 5,000IU |
Rifampicin | 5.0mg | 10.0mg |
Trimethoprim | 5.0mg | 10.0mg |
Amphoteracin B | 5.0mg | 10.0mg |
Directions (to prepare Preston Campylobacter Selective Agar)
Suspend 18.5g of Campylobacter Agar Base in 475ml of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml of Lysed Horse Blood (SR0048), and 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) reconstituted as directed and one vial of Campylobacter Growth Supplement (SR0232). Mix well and pour into sterile Petri dishes.
Directions (to prepare Preston Campylobacter Selective Enrichment Broth)
Dissolve 12.5g of Nutrient Broth No.2 (CM0067) in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood (SR0048), 1 vial of Preston Campylobacter Selective Supplement (SR0117 or SR0204) and 1 vial of Campylobacter Growth Supplement (SR0232). Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C.
It is essential that the head space above the liquid should be as small as possible to ensure microaerobic conditions.
Description
The Preston Campylobacter Selective Agar is based on the formulation described by Bolton and Robertson1. This medium was specifically formulated to be suitable for isolation of Campylobacter species from all types of specimens (human, animal, avian and environmental).
In comparative studies2 of the selective media of Skirrow, Butzler, Blaser, Campy-BAP and Preston, the Preston medium was found to give the maximum isolation rate of Campylobacter species from all types of specimens tested and also to be the most selective.
Oxoid Campylobacter Agar Base has been prepared from materials described by Bolton and Robertson1. It is suitable as a basal medium for the selective supplements of Blaser-Wang, Skirrow and Butzler.
Preston Campylobacter Selective Supplement (SR0117) can also be used to prepare Preston Campylobacter Selective Enrichment Broth2.
The selective enrichment technique is recommended for specimens and food samples that are expected to be heavily contaminated and/or carry small numbers of viable colony forming units. The Preston Campylobacter Selective Enrichment Broth which is supplemented with the growth supplement SR0232, made to the formulation of George et al.3 effectively quenches toxic compounds which may form on exposure of the medium to light and air4.
Technique
Direct Selective Plating Method
† When few Campylobacter colony forming units are present 48 hours incubation is necessary.
Selective Enrichment Broth Technique
CAMPYLOBACTER TRANSPORT MEDIUM
Campylobacter Selective Supplement (Preston) is incorporated in an improved medium for storage and transportation of thermophilic Campylobacters 5.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Agar - Straw coloured gel
Broth - Straw coloured solution
Quality control
Positive control: | Expected results |
Campylobacter jejuni ATCC® 29428 * | Good growth; grey-brown coloured colonies |
Negative control: | |
Escherichia coli ATCC® 25922 * | Inhibited |
References
1. Bolton F.J. and Robertson L. (1982) J. Clin. Pathol. 35. 462-467.
2. Bolton F.J., Coates D., Hinchliffe P.M. and Robertson L. (1983) J. Clin. Pathol. 36. 78-83.
3. George H.A., Hoffman P.S., Kreig N.R. and Smibert R.M. (1979) Can. J. Microbiol. 25. 8-16.
4. Bolton F.J., Coates D. and Hutchinson D.N. (1984) J. Appl. Bact. 56. 151-157.
5. Rogol M., Schnaidman B., Katzenelso E. and Sechter I. (1990) Eur. J. Clin. Microbiol. Inf. Dis. 9. 760-762.