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Material Safety Data Sheet

Optional Products

Organisms

Organisms this product works with:

Dehydrated Culture Media

M17 BROTH

Code: CM0817

For improved growth of lactic streptococci and their bacteriophages.

Typical Formula*

gm/litre

Tryptone

5.0

Soya peptone

5.0

`Lab-Lemco’ powder

5.0

Yeast extract

2.5

Ascorbic acid

0.5

Magnesium sulphate

0.25

Di-sodium-glycerophosphate

19.0

pH 6.9 ± 0.2 @ 25°C

 

* Adjusted as required to meet performance standards 

Directions
Suspend 37.25g in 950ml of distilled water and bring gently to the boil. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile lactose solution (10% w/v).

Lactose solution 10% (w/v)
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2mm membrane.

Description
M17 Broth has been produced in parallel with M17 Agar CM0785. Its use in conjunction with M17 Agar in bacteriophage assays has been described by Terzaghi and Sandine1. These workers also suggest that M17 Broth would be a suitable medium for the maintenance of starter cultures because of its considerable buffering capacity and the little effect it has on the subsequent acid-producing ability of these cultures.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured solution

Positive controls:

Expected results

Streptococcus thermophilus ATCC® 14485

Turbid growth

Negative control:

 

Lactobacillus bulgaricus ATCC® 11842

No growth

References
1. Terzaghi B. E. and Sandine W. E. (1975) Applied Microbiology 29. 807-813.
2. Anderson A. W. and Elliker P. R. (1953) J. Dairy Science 36. 161-167.
3. Reiter B. and Oram J. D. (1962) J. Dairy Res. 29. 63-77.
4. Shankar P. A. and Davies F. L. (1977) J. Soc. Dairy Technology 30. 28-30.
5. International Dairy Federation (1981) Joint IDF/ISO/AOAC Group E44.
6. International Organization for Standardization (1985) ISO/DIS 7889.

 
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