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Dehydrated Culture Media

TRYPTONE SOYA BROTH (USP)

Code: CM0876

A highly nutritious general purpose medium for the growth of bacteria and fungi. The constituents have been specially selected for their filterability making this formulation particularly suitable for Media Fill Tests carried out in the Pharmaceutical industry.

Formula
gm/litre
Pancreatic digest of casein
17.0
Papaic digest of soybean meal
3.0
Sodium chloride
5.0
Dibasic potassium phosphate
2.5
Glucose
2.5
pH 7.3 ± 0.2  

Directions
Add 30 g to 1 litre of distilled water, mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Description
A highly nutritious and versatile medium recommended for general laboratory use. Due to the inclusion of both Tryptone and Soya Peptone, the medium will support a luxuriant growth of many fastidious organisms without the addition of serum, etc.
Oxoid Tryptone Soya Broth conforms to formulations detailed in various international pharmacopoeia and is suitable for use in Media Fill Tests1,4,5,6.

Technique
Aerobic Cultivation.
Tryptone Soya Broth may be used for the cultivation of aerobes and facultative anaerobes, including some fungi. Cultures should be examined at frequent intervals, as maximum growth is reached earlier than with less nutritious media and the phase of decline consequently begins sooner.
Anaerobic Cultivation
The addition of a small amount of agar renders the broth suitable for the cultivation of obligatory anaerobes, such as Clostridium species. For this purpose, the broth (with added agar) should be used soon after sterilisation or heated and cooled just before inoculation.
Blood Culture
The superior growth-promoting properties of Tryptone Soya Broth make it especially useful for the isolation of organisms from blood or other body fluids. Anticoagulants such as `liquoid’† (sodium polyanethyl sulphonate) or sodium citrate may be added to the broth prior to sterilisation. Five to 10 ml of blood may be added to 50 ml of medium.
Tryptone Soya Broth is especially suitable for the tube dilution method of antibiotic susceptibility testing.
Oxoid laboratory tests have shown that Tryptone Soya Broth has a greater ability to resuscitate heated spores of Bacillus stearothermophilus than Dextrose Tryptone Broth. Tryptone Soya Broth is recommended in the USP XXIV (Soybean Casein Digest Medium) for the recovery of organisms after sterilisation processes1. A positive result is revealed after 24-48 hours incubation at 55°C, by a heavy growth of organisms causing turbidity in the broth.
† Roche Products Ltd. Welwyn Garden City, Herts.

Selective Culture Media
Tryptone Soya Broth is used in food bacteriology as the basal medium that a variety of selective agents are added to for selective enrichment of Staphylococcus aureus2and Escherichia coli O1573.

Storage Conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

Appearance
Dehydrated Medium: Straw coloured, free-flowing powder.
Prepared medium: Straw coloured solution.

Quality control

Positive controls: Expected results
Streptococcus pneumoniae ATCC® 6305 * Turbid growth
Staphylococcus aureus ATCC® 25923* Turbid growth
Negative control:  
Uninoculated medium. No change

* This organism is available as a Culti-Loop®

References
1. US Pharmacopoeia. XXIV. (2000) Bethesda MD.
2. Compendium of Methods for the Microbiological Examination of Foods. 3rd edition. Vanderzant C. and Splittstoesser D.F. (eds). APHA. Washington DC.
3. Practical Food Microbiology. Roberts D., Hooper W and Greenwood M. (Eds). Public Health Laboratory Service, London 1995.
4. British Pharmacopoeia Volume II (2000)
5. European Pharmacopoeia . 3rd Edition (2001)
6. Japanese Pharmacopoeia. 12th Edition. (2000)

 

 
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