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Dehydrated Culture Media

ANAEROBE BASAL BROTH

Code: CM0957

A nutrient broth for the growth of anaerobic micro-organisms, particularly Bacteroides spp. and other fastidious anaerobes.

Typical Formula*

gm/litre

Peptone

16.0

Yeast extract

7.0

Sodium chloride

5.0

Starch

1.0

Dextrose

1.0

Sodium pyruvate

1.0

Arginine

1.0

Sodium succinate

0.5

L-cysteine HCl

0.5

Sodium bicarbonate

0.4

Ferric pyrophosphate

0.5

Haemin

0.005

Vitamin K

0.0005

Sodium thioglycollate

0.5

Dithiothreitol
1.0

pH 6.8 ± 0.2 @ 25°C

 
* Adjusted as required to meet performance standards 

Directions
Suspend 35.4g of Anaerobe Basal Broth in 1 litre of distilled water. Bring to the boil to dissolve. Distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Oxoid Anaerobe Basal Broth is formulated from a range of nutrients which have been selected to optimise the recovery and growth of the majority of anaerobic organisms of clinical importance.

The formulation includes yeast extract as a source of vitamins and starch is included to absorb toxic products1. Sufficient arginine is added to ensure growth of Eubacterium lentum 2, whilst haemin and vitamin K are present as they are essential for the growth of Bacteroides spp.3. Pyruvate is present as an energy source for asaccharolytic cocci such as Veillonella spp. It also eliminates traces of hydrogen peroxide which may be produced by the action of molecular oxygen on medium constituents4. Sodium succinate improves the growth of Prevotella melaninogenica and Bacteroides spp5. It is included together with the reducing agent L-cysteine hydrochloride, which has been shown to stimulate directly the growth of some anaerobes6.

Technique
It is preferable to use freshly reconstituted and sterile medium which is inoculated as soon as it has cooled to room temperature. Tubes which are not used on the day of preparation should be placed in a boiling water bath or steamer for approximately 15 minutes to remove dissolved oxygen. They should be allowed to cool without agitation and then inoculated. Incubate broths in an appropriate atmosphere.

Storage conditions and Shelf life
Anaerobe Basal Broth should be stored tightly capped in the original container at 10-30°C. When stored as directed, the medium will remain stable until the expiry date printed on the label.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw/green coloured solution

Quality control

Positive controls:

Expected results

Peptostreptococcus anaerobius ATCC® 27337*

Turbid growth

Prevotella loescheii ATCC 15930

Turbid growth

Negative control:

 

Uninoculated medium

No change

* This organism is available as a Culti-Loop®

.References
1. Ajello GW. Feeley JC. Hayes PS. et al. Trans-isolate medium: a new medium for primary culturing and transport of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. J. Clin. Micro. 1984:20:55-8.
2. Sperry JF. Wilkins TD. Arginine, a growth-limiting factor for Eubacterium lentum. J. Bacteriol 1976:127:780-4.
3. Gibbons RJ. and MacDonnald JB. Haemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J. Bact. 1960:80:164-170.
4. Neilson PA. Role of reduced sulphur compounds in nutrition of Proprionobacterium acnes. J. Clin. Micr. 1983:17:276-9.
5. Lev M. Keudell KC. and Milford AF. Succinate as a growth factor for Bacteroides melaninogenicus. J. Bact. 1971:108:175-8.
6. Shanson DC. and Singh J. Effect of adding cysteine to brain-heart infusion broth on the isolation of Bacteroides fragilis from experimental blood cultures. J. Clin. Path. 1981:34:221-3.

 
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