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Material Safety Data Sheet

Other Items

Either of the following items may be used together with this product:

Organisms

Organisms this product works with:

Dehydrated Culture Media

ARCOBACTER BROTH

Code: CM0965

An enrichment broth for Arcobacter species

Typical Formula*

gm/litre

Peptone

18.0

Yeast extract

1.0

Sodium chloride

5.0

pH 7.2 ± 0.2 @ 25°C

 

* Adjusted as required to meet performance standards

Directions
Dissolve 12g of Arcobacter Broth in 500ml of distilled water. Sterilise at 121°C for 15 minutes and allow to cool to below 50°C

For use with CAT supplement (SR0174)
Add one vial per 500ml medium of CAT Selective Supplement reconstituted as directed. Dispense into sterile containers.

For use with CCDA supplement (SR0155)
Add one vial per 500ml medium of CCDA Selective Supplement reconstituted as directed. Dispense into sterile containers.

Inoculated plates should be incubated aerobically, at 30°C, for 24 hours.

Description
Oxoid Arcobacter Broth is intended for use with Cefoperazone, Amphotericin B, Teicoplanin (CAT) Selective Supplement (SR0174) as a selective enrichment broth for the growth of Arcobacter species and with the more selective CCDA (SR0155) for the selective enrichment of Arcobacter butzleri.

Peptones in the base medium are specifically designed to provide the ideal growth conditions for Arcobacter species. The incubation conditions and the absence of blood or charcoal supplements suppress the growth of Campylobacter species. Cefoperazone, Amphotericin B and Teicoplanin are added to suppress the growth of competing flora, but allow the growth of Arcobacter species. CCDA Selective Supplement SR0155 is substituted for CAT to selectively isolate Arcobacter butzleri1,2.

Arcobacters are micro aerophilic, Gram-negative rods, which were formerly classified as Campylobacter3. Four Arcobacter species have been identified: A. butzleri, A. cryaerophilus, A. skirrowii and A. nitrofigilis, all of which have a greater propensity to grow in air than Campylobacter spp.
Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii have been associated with disease in humans4,5, and typically are isolated from faecal samples.

Arcobacter butzleri has been isolated from patients with bacteraemia, peritonitis, endocarditis and diarrhoea. Patients with Arcobacter butzleri-associated diarrhoea typically suffer from abdominal pain and nausea, fever, chills, vomitting and malaise, but the organism has also been implicated in an outbreak of recurrent abdominal cramps without diarrhoea6. The source of infection is usually contaminated water or sewage6.
Arcobacter cryaerophilus group 1B has been isolated from patients with bacteraemia and diarrhoea4,5, although it is a much less common human isolate than Arcobacter butzleri6.

Arcobacter nitrofigilis has only been isolated from marsh grass to date, never from humans or animals. It is not thought to be clinically significant7.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured solution

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Quality control

Positive control: Expected Results

Arcobacter butzleri ATCC® 49616

 Colourless colonies

Negative control:

 

Escherichia coli ATCC® 25922

 Inhibited


References
1. Data on file
2. Lammerding, A.M., Harris, J.E., Lior, D.L. et al. Presented at the 81st annual meeting of IAMFES (1994).
3. Vandamme, P., Falsen, E., Rossau, R., Hoste, B., Segers, P., Tytgat, R., De Ley, J. (1991). Int. J. Syst. Bacteriol. 41:88-103.
4. Kiehlbauch, J.A., Brenner, D.J., Nicholson, M.A., Baker, C.N., Patton, C.M., Steigerwalt, A.G., Wachsmuth, I.K. (1991). J. Clin. Microbiol. 29:376-385.
5. Vandamme, P., Vancanneyt, M., Pot, B., Mels, L., Hoste, B., Dewettinck, D., Vlaes, L., Van den Borre, C., Higgins, R., Hommer, J. (1992). Int. J. Syst. Bacteriol. 42:344-356.
6. Vandamme, P., Pugina, P., Benzi, G., Van Etterick, R., Vlaes, L., Kersters, K., Butzler, J., Lior, H., Lauwers, S. (1992). J. Clin. Microbiol. 30:2335-2337.
7. Atabay, H.I and Corry, J.E.L. (1998) Int. J. Food Microbiol, 41, 53-58.

 
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