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Dehydrated Culture Media

SODIUM CHLORIDE PEPTONE BROTH (BUFFERED)

Buffered Sodium Chloride Peptone Solution pH 7.0 (Ep/Usp/Jp/Bp)

CODE: CM0982

an isotonic diluent made to the harmonised Microbial Limit Testing chapters from the EP/USP/JP specification

Typical Formula

gm/litre

Potassium dihydrogen phosphate

3.56

Disodium hydrogen phosphate anhydrous

5.77

Sodium chloride

4.3

Peptone (meat)

1.0

pH 7.0 ± 0.2 @ 25°C

 
5.77 of disodium hydrogen phosphate anhydrous are equivalent to 7.2g of disodium hydrogen phosphate dihydrate

Directions
Suspend 14.63g of Sodium Chloride Peptone Broth (Buffered) in 1 litre of water (purified as required). If required, add 1-10ml Tween® 20 or 80 per litre of medium. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Description
Sodium Chloride Peptone Broth (Buffered) is intended for the dilution of samples in the analysis of non-sterile products for microbial contaminants.

The combination of phosphate buffer, sodium chloride and peptone increases the viability of sensitive micro-organisms by providing an isotonic environment. This is of particular importance when trying to recover cells that may be stressed or sensitive to osmotic pressure.

This solution conforms to the harmonised Microbial Limit Testing chapters from the EP/USP/JP 1,2,3.  Additional information about this and other products in our harmonised range can be obtained by downloading our Harmonised Culture Media Portfolio.

Storage conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared media at room temperature.

Appearance
Dehydrated medium: Light straw coloured, free-flowing powder
Prepared medium: Light straw coloured solution

Quality control

Micro-organismsExpected results
Escherichia coli ATCC® 8739*Colony counts of <100 CFU at times zero, 2 hours and 24 hours if stored at 2-8°C
Staphylococcus aureus ATCC® 6538*
Pseudomonas aeruginosa ATCC® 9027*
Aspergillus brasiliensis ATCC® 16404*

*This organism is available in a Quanti-Cult™ format

References
1. US Pharmacopoeia 30 (2008)
2. European Pharmacopoeia 6.1. 2.6.12 Microbiological Examination of Non-Sterile Products: Harmonised Method: Microbial Enumeration tests 2.6.13 Microbiological Examination of Non-Sterile Products: Test for Specified Micro-organisms. B. Harmonised Method. (2008).
3. Japanese Pharmacopoeia. 15 th Edition. (2006)

 
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