Organisms in the Industrial sector this product works with:
Other products used in the isolation of Listeria monocytogenes :
24 LISTERIA ENRICHMENT BROTH (24 LEB)
24 LEB Base
Code: CM1107
This medium is for the selective enrichment of Listeria monocytogenes and other Listeria spp. from food and environmental samples. Only for use with BAX® System PCR L. monocytogenes 24E and BAX® System PCR Genus Listeria 24E assays.
Typical Formula* | gm/litre |
Peptone | 23.4 |
Yeast extract | 5.0 |
Lithium chloride | 10.0 |
Ferric ammonium citrate | 0.1 |
Sodium chloride | 5.0 |
pH 7.4 ± 0.2 @ 25°C |
|
24 LEB Selective Supplement
Code: SR0243
vial contents | SR0243E | per litre before |
Polymyxin | 5.0mg | 10.0mg |
Quinolone antimicrobials | 17.5mg | 35.0mg |
Directions
Completely dissolve 21.75g of 24 LEB Base per 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add the contents of 1 vial of 24 LEB Selective Supplement per 500ml medium, reconstituted as directed. Cool to room temperature before use, and, once made, store the medium at 2-8°C in the dark.
Technique
Refer to BAX® System user guide and product inserts for PCR Assay L. monocytogenes 24E (QB8125C) and BAX® System PCR Assay Genus Listeria 24E (QB8135C) for instructions for use.
Quality control
Positive controls: | Expected results |
Listeria monocytogenes ATCC®13932 | Turbid growth |
Listeria innocua ATCC® 33090* | Turbid growth |
Negative controls: | |
Enterococcus faecalis ATCC® 29212* | No growth/inhibited growth |
Escherichia coli ATCC® 25922* | No growth |