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Dehydrated Culture Media

24 LISTERIA ENRICHMENT BROTH (24 LEB)

24 LEB Base

Code:  CM1107

This medium is for the selective enrichment of Listeria monocytogenes and other Listeria spp. from food and environmental samples. Only for use with BAX® System PCR L. monocytogenes 24E and BAX® System PCR Genus Listeria 24E assays.

Typical Formula*  

gm/litre

Peptone 

23.4

Yeast extract 

5.0

Lithium chloride

10.0

Ferric ammonium citrate

0.1

Sodium chloride

5.0

pH 7.4 ± 0.2 @ 25°C

 

* Adjusted as required to meet performance standards 

24 LEB Selective Supplement

Code: SR0243 
    

vial contents

SR0243E
(1 vial per
500ml medium

per litre before
sample addition

Polymyxin

5.0mg

10.0mg

Quinolone antimicrobials

17.5mg

35.0mg

 

Directions
Completely dissolve 21.75g of 24 LEB Base per 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add the contents of 1 vial of 24 LEB Selective Supplement per 500ml medium, reconstituted as directed. Cool to room temperature before use, and, once made, store the medium at 2-8°C in the dark.

Technique
Refer to BAX® System user guide and product inserts for PCR Assay L. monocytogenes 24E (QB8125C) and BAX® System PCR Assay Genus Listeria 24E (QB8135C) for instructions for use.

Quality control

Positive controls:Expected results
Listeria monocytogenes ATCC®13932Turbid growth
Listeria innocua ATCC® 33090*Turbid growth 
Negative controls: 
Enterococcus faecalis ATCC® 29212* No growth/inhibited growth 
Escherichia coli ATCC® 25922* No growth 

* This organism is available as a Culti-Loop®
 
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