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Dehydrated Culture Media

Linden Grain Medium

Code: CM1152

A medium for media fill process simulation for beverage bottling, to test for low acid beverage spoiling bacteria

Typical Formula*

gm/litre

Glucose

20

Yeast extract

3.5

Casein peptone

2

Ammonium sulphate

2

Potassium di-hydrogen phosphate

1

Magnesium sulphate

1

TOTAL

29.5g/L

pH 4.2 ±  0.2 at 25ºC

 

* Adjusted as required to meet performance standards 

Directions

Suspend 29.5g in 1 litre of distilled water. Boil to dissolve the medium completely. Sterilize at 121°C for 15 minutes.

Technique

The Linden Grain Medium is designed for media fill process simulation for beverage bottling (also known as aseptic conditioning testing) where the broth is prepared directly in product preparation tanks, processed in a heat exchanger and filled into cans or bottles. Usually a challenge test is conducted, so many litres of broth are prepared at one time.

The medium permits the growth of organisms that can spoil low acid beverages. Presence of these organisms is indicated by turbid growth in the broth.

Storage conditions and Shelf life

Store the dehydrated medium at 10–30°C and use before the expiry date on the label.

Appearance

Dehydrated medium: Straw coloured powder

Quality control

Organism:

Expected results

Candida albicans

ATCC® 10231*

Turbid growth

Saccharomyces cerevisae

ATCC® 9763*

Turbid growth

Aspergillus brasiliensis

ATCC® 9642*           

Turbid growth

Aspergillus brasiliensis

ATCC® 16404*

Turbid growth

*This organism is available as a Culti-Loop®

 
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