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Material Safety Data Sheet

Required Products

Items which require this product:

Organisms

Organisms this product works with:

For this Organism

Other products used in the isolation of Campylobacter:

Atmosphere Generation System

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CAMPYGEN COMPACT

Code: CN0020

Description
CampyGen Compact for 1 or 2 Petri dishes, is a simple system for generating microaerobic conditions. The system consists of a plastic pouch and sealing clip and a paper gas generating sachet. The paper sachet contains ascorbic acid which reacts on contact with air to produce the microaerobic conditions for the growth of microaerophilic organisms.

Components
20 CampyGen Compact paper sachets, individually wrapped in foil
1 product leaflet

Materials Required but not Provided
Sealing Clips (AN0005)
Plastic Pouches (AG0020)
W-Zip Plastic Pouches (AG0060)

Precautions
This product is for in vitro use only. The CampyGen Compact paper sachet will become active on contact with air. It is essential that the plastic pouch is sealed within one minute of exposing the paper sachet to the air. The reaction of ascorbic acid with oxygen is exothermic. However, the temperature of the CampyGen Compact paper sachet will not exceed 65°C.

Storage
Store at 2-25°C. Under these conditions, the CampyGen Compact sachets will retain their activity until the expiry date given on the outer box and on the foil wrap of the sachets.

Directions
1
Place 2 inoculated plates in a plastic pouch. Disposable plastic Petri dishes should be of the vented variety to aid gas transfer between the interior and exterior of the plates. If only one plate is to be inoculated, an uninoculated plate should also be placed in the plastic pouch to prevent further activity as the volume of O2 and CO2 is critical.
2 Tear open a CampyGen Compact foil sachet at the tear-nick indicated. Remove the CampyGen Compact paper sachet from within.
3 Immediately place the paper sachet in the plastic pouch with the plates. N.B. The paper sachet will become warm to the touch on exposure to air.
4 Expel excess air from the plastic pouch. Seal the plastic pouch immediately with a sealing clip or w-zip closure as appropriate. The time taken between opening the foil sachet and sealing the plastic pouch should not exceed 1 minute.
5 Incubate appropriately.
6 After the incubation period, remove the plates and examine for the presence of colonies. If the plates require re- incubation, a fresh CampyGen Compact sachet must be used, following steps 2-5 described above. N.B. The plates may be initially inspected through the transparent plastic pouch. If the bag is opened, a fresh CampyGen Compact sachet is required for re-incubation.
7 After incubation, the exhausted CampyGen Compact paper sachet and plastic pouch should be sterilised and discarded with the non-hazardous laboratory waste.

Control Testing
The user should check their technique periodically for the ability to provide adequate conditions for the growth of microaerophilic bacteria.
Campylobacter jejuni
ATCC® 33291 may be used for this purpose.

Disposal
On removal from the pouch after incubation, the CampyGen Compact paper sachet will retain a small amount of activity and become warm. The sachets should be allowed to cool to room temperature prior to sterilisation and disposal with the non-hazardous laboratory waste.

 
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