Part of Thermo Fisher Scientific
Material Safety Data Sheet
Diagnostic Discs
Codes: DD0014 and DD0015
For the differentiation of vibrios from other Gram-negative rods.
Contents
DD0014 1 cartridge. containing 50 discs. Disc content 0129 10 mg
per disc.
DD0015 1 cartridge. containing 50 discs. Disc content 0129 150 mg
per disc.
Description
The sensitivity of vibrios to the vibrio static agent 0129 (2,4-Diamino-6,7-di-iso-propylpteridine
phosphate) has long been recognised1. This test is of great value
in differentiating vibrios from other Gram-negative rods and particularly from
aeromonads, which are characteristically resistant to 0129. The degree of sensitivity
of vibrios to 0129 can also be used as a diagnostic feature in differentiation
of Vibrio species (see Table 1).
Negative 0129 tests must be interpreted with caution. Strains of Vibrio cholerae
resistant to 0129 and trimethoprim have been reported2. Both plasmids
and transposons appear to be responsible3.
Specialised sensitivity testing media which contain low levels of sodium chloride
should not be used as these may give poor growth of halophilic vibrios and
may also give false positive results with Enterobacteriaceae4.
Nutrient Agar CM0003 can be recommended for such testing.
Table 1* Sensitivity of Vibrio Species to 0129
|
MIC (µg/ml) |
Disc Test |
|
|
Vibrio parahaemolyticus |
R |
S |
|
Vibrio alginolyticus |
R |
S |
|
Vibrio fluvalis |
R |
S |
|
Vibrio furnissi |
R |
S |
|
Aeromonas |
R |
R |
|
Vibrio |
R |
R |
|
Vibrio |
R |
R |
|
Vibrio |
S |
S |
|
Vibrio |
S |
S |
|
Vibrio |
S |
S |
Technique
1 Evenly incubate the surface of a Nutrient Agar plate CM0003.
2 Place one DD0014 and one DD0015 disc on each plate.
3 Incubate at 35° C for 24 hours.
4 Observe for zones of inhibition.
Quality Control
Use known strains of 0129 sensitive and resistant organisms to monitor the
performance of the discs.
References
1 Shewan J. M. and Hodgkiss W. (1954) Nature 63. 208-209.
2 The Lancet, August 8th 1992, 340. 366-367.
3 Gerbaud G., Dodin A., Goldstein F. and Courvain P. (1985) Ann. Inst. Past./Microbiol.
136B. 265-273.
4 Furniss A. L., Lee J.V. and Donovan T.J. (1978) `The Vibrios’. Public Health
Laboratory Service, Monograph Series. Number 11.
5
BSOP ID 19 Standards unit, Evaluations and Standards Laboratory Identification
of Vibrio species 2005.
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