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Code: DD0016

Sodium polyanethol sulphonate discs for the presumptive identification of Peptostreptococcus anaerobius.

Contents
1 cartridge. containing 50 discs.

Disc Content

Description
Wideman et al.¹ reported that Peptostreptococcus anaerobius may account for one-fifth to one-third of all Gram-positive cocci encountered in clinical specimens and confirmed that all strains of Peptostreptococcus anaerobius are totally inhibited by sodium polyanethol sulphonate (SPS) as described previously by Graves et al.². It has been recommended¹ that the SPS disc method should be used as a rapid and simple method for the presumptive identification of Peptostreptococcus anaerobius. By this method all strains of Peptostreptococcus anaerobius give inhibition zones of 12-30 mm. Peptostreptococcus micros and Peptococcus prevotii may give zones that overlap the zone size range obtained with Peptostreptococcus anaerobius.

SPS Disc Sensitivity of Clinical Isolates¹

^a Two isolates had zone diameters of 10 mm and one isolate had a 12 mm zone diameter.
^b One isolate had a 17 mm zone of diminished growth.
^c One isolate had a zone of 16 mm.

Technique
1 Prepare Wilkins-Chalgren Anaerobe Agar CM0619 plates as directed.
2 Adjust the 18-24 hour culture of the test organism to give a 0.5 McFarland standard.
3 Evenly inoculate the surface of a Wilkins-Chalgren Anaerobe Agar plate with the culture under test.
4 Place one DD0016 on the plate.
5 Incubate anaerobically at 35°C for 48 hours.
6 Observe zones of inhibition.

Quality Control
Use known strains of Peptostreptococcus anaerobius to monitor the performance of the discs.

References
1 Wideman P. A., Vargo V. L., Citronbaum D. and Finegold S. M. (1976) J. Clin. Micro. 4. 330-333.
2 Graves M. H., Morello J. A. and Kocka F. E. (1974) Appl. Microbiol. 27. 1131-1133.