Diagnostic Reagents

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VDRL TEST KIT

Code: DR0525

A test for the detection and quantitative assessment of reagin antibodies in syphilis screening.

Introduction
The Oxoid VDRL Carbon Antigen Test is a macroscopic non-treponemal flocculation test for use in the detection and quantification of reagin antibodies. A presumptive diagnosis of syphilis can be made when these antibodies are detected in serum. The Card Test uses a modified form of the VDRL antigen1 containing micro-particulate carbon to improve the reading of results.
The test can be performed using unheated serum or plasma. The VDRL Carbon Antigen Suspension can also be used on both single- and multi-channel Auto-Analyzer equipment.2,3,4,5,6

Components of the Kit DR0525 / DR0526
DR0527 VDRL Carbon Antigen Suspension.
DR0528 Positive Control Serum.
DR0529 Negative Control Serum.
Test Cards.
Mixing Sticks.
Instruction leaflet.

Precautions
The human sera used in the manufacture of the controls were shown to be negative for HbsAg (Hepatitis B surface antigen), Hepatitis C and HIV 1 and 2 antibodies by FDA approved tests. However, this cannot guarantee the absence of virus, and therefore the reagents should be handled as capable of transmitting hepatitis.
The positive and negative control sera contain sodium azide (0.1%). The VDRL Carbon Antigen Suspension contains thiomersalate (0.1%).

Storage
The VDRL Carbon Antigen Suspension must be stored at 2-8°C. Under these conditions it will retain its reactivity until the date shown on the label. Do not freeze the reagent.
The positive and negative control sera can be divided into 0.1 ml amounts and stored at -20°C.
Store test cards at ambient temperature; use each card once and discard. Take care not to finger mark the circled areas.

References
1. Portnoy J, Brewer J.H. and Harris A.D.(1962) U.S. Public Health Report 77. 645
2. McGrew B.E., Stout G. W. and Falcone V.H. (1968) Am. J.Med. Tech 34. 634
3. McGrew B.E., Ducross M.J.F., Stout G.W. and ] Falcon V.H. (b) Am.J.Cain.Pathos. 50. 52
4. Narrowness L.C. (1968) Automation in Analytical Chemistry, Technician Symposium 1967 1.157 New York Mediad
5. Sevens R.W. and Strobil E. (1970) Am.J.Cain. Pathos. 53. 32
6. Stout G.W., McGrew B.E. and Falcon V.H.(1968) Conf.Public Health Lab.Directors 26. 7

 
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