Part of Thermo Fisher Scientific
Organisms this product works with:
Other products used in the isolation of Staphylococcus sp:
Code: DR0850
Staphytect Plus is a latex slide agglutination test 1 for the differentiation of Staphylococcus aureus by detection of clumping factor, Protein A and certain polysaccharides found in methicillin resistant Staphylococcus aureus (MRSA) from those Staphylococci that do not possess these properties.
Introduction
Traditionally, differentiation between coagulase-positive and coagulase-negative
staphylococci has been performed either with the tube coagulase test that
detects
extracellular staphylocoagulase or the slide coagulase test that detects the
clumping factor (bound coagulase) present on the bacterial cell surface. Several
other differentiation tests are also available including the passive haemagglutination
test (Oxoid Staphylase DR0595) and the DNase test.
It has been reported that approximately 97% of human strains of Staphylococcus
aureus possess both bound coagulase and extracelluar staphylocoagulase.
Protein A is found on the cell surface of about 95% of human strains of Staphylococcus
aureus and has the ability to bind the Fc portion of immunoglobulin G (IgG)
2 .
It has been observed that certain methicillin-resistant strains of Staphylococcus aureus
(MRSA) may express undetectable levels of clumping factor and Protein A3,4,5
. It has been shown however that these strains all possess capsular polysaccharide 6.
The capsule can mask both Protein A and the clumping factor thereby preventing
agglutination.
Staphytect Plus uses blue latex particles coated with porcine fibrinogen and
rabbit IgG including specific polyclonal antibodies raised against capsular
polysaccharides of Staphylococcus aureus 7,8.
When the reagent is mixed on a card with colonies of Staphylococcus
aureus,
rapid agglutination occurs through the reaction between (i) fibrinogen and
clumping
factor, (ii) Fc portion of IgG and Protein A (iii) specific IgG and capsular
polysaccharide. Agglutination may also occur with other species which possess
clumping factor or Protein A such as Staphylococcus hyicus and Staphylococcus
intermedius. If neither clumping factor, Protein A or specific capsular
polysaccharides are present, agglutination will not occur and the result
will
be regarded as negative. The most frequent coagulase and Protein A negative
isolates of staphylococci are Staphylococcus epidermidis.
Components of the Kit
DR0851 Staphytect Plus Test Reagent (5.6 ml)
Blue latex particles coated with both porcine, fibrinogen and rabbit IgG together
with specific polyclonal antibodies raised against capsular polysaccharide of
Staphylococcus aureus. Each bottle contains sufficient reagent for 100 tests.
DR0852 Staphytect Plus Control Reagent (5.6 ml)
Blue unsensitised latex particles. Each bottle contains sufficient reagents
for 100 tests.
DR0500 Reaction Cards
There are 35 disposable reaction cards provided in the kit
Instruction Leaflet
Materials required but not provided:
Timer
Microbiological Loop
A suitable laboratory disinfectant
Positive Control: Staphylococcus aureus strain such as ATCC®25923.
Negative Control: Staphylococcus epidermidis strain such as ATCC®12228.
Storage
This kit must be stored at 2-8°C away from direct sunlight or heat
sources. Do not Freeze.
The
kit should not be used after the expiry date printed on the outside of the
carton.
Precautions
Reagents contain 0.095% sodium azide as a preservative. Sodium azide is toxic
and may react with lead or copper plumbing to produce metal azides which are
explosive by contact detonation. To prevent azide accumulation in plumbing flush
with copious amounts of water immediately after waste disposal.
Specimen materials may contain pathogenic organisms handle with appropriate
precautions.
References:
1. Essers, L. and Radebold, K. (1980). "Rapid and Reliable Identification
of Staphylococcus aureus by a Latex Agglutination Test". J.Clin.Microbiol.
12: 641-643.
2. Taussig, M.J. (1984). Processes in Pathology and Microbiology 2nd Ed.
520-530. Blackwell,Oxford.
3. Ruane, P.J., Morgan, M.A., Citron, D.M. and Mulligan, M.E. (1986). "Failure
of Rapid Agglutination Methods to Detect Oxacillin-Resistant Staphylococcus
aureus". J.Clin.Microbiol. 24: 490-492.
4. Roberts, J.I.S. and Gaston, M.A. (1987). "Protein A and coagulase expression
in epidemic and non-epidemic Staphylococcus aureus". J.Clin.Pathol. 40:
837-840.
5. Wanger, A.R., Morris, S.L. Ericsson, C., Singh, K.V. and LaRocco, M.T. (1992).
"Latex Agglutination-Negative Methicillin-Resistant Staphylococcus aureus
Recovered from Neonates: Epidemiologic Features and Comparison of Typing Methods".
J.Clin. Microbiol. 30: 2583-2588.
6. Fournier, J.M., Boutonnier, A. and Bouvet, A. (1989). "Staphylococcus
aureus Strains Which Are Not Identified by Rapid Agglutination Methods Are
of Capsular Serotype 5". J.Clin.Microbiol. 27: 1372-1374.
7. Fournier, J.M., Bouvet, A. Boutonnier, A., Audurier, A. , Goldstein, F.,
Pierre, J., Bure, A., Lebrun, and L., Hochkeppel, H.K. (1987). Predominance
of Capsular Polysaccharide Type 5 among Oxacillin-Resistant Staphylococcus
aureus". J.Clin.Microbiol. 25: 1932-1933.
8. Karakawa, W.W., Fournier, J.M., Vann, W.F., Arbeit, R., Schneerson, R.S.,
and Robbins, J.B. (1985). "Method for the Serological Typing of the Capsular
Polysaccharides of Staphylococcus aureus ". J.Clin.Microbiol.
22: 445-447.
9. Kloos, W.E., Jorgensen J.H.(1988). Staphylococci. pp.143-153. In
Manual of Clinical Microbiology. 4th Edn. (Eds) Lennette, E.H., Balows, A.Hauser
W.J., and Shadomy, H.J. Assoc.Amer.Microbiol. Washington.
10. Data on file at Oxoid Ltd.
11. Jean-Pierre, H., Darbas, H., Jean-Roussenq,. A. & Boyer, G.(1989). "Pathogenicity
in Two Cases of Staphylococcus schleiferi, a Recently Described Species".
J.Clin.Microbiol.27: 2110-2111.
12. Frenney, J., Brun, Y., Bes, M., Meugnier H., Grimont, F., Grimont, P.A.D.,Nervie,
C., & Fleurette, J. (1988). "Staphylococcus schleiferi sp. nov. Two
species from Human Clinical Specimens". Int.J.Sup Bacteriol. 38: 168-172.
13. Phillips, W.E., and Kloos, W.E. (1981). "Identification of Coagulase-Positive
Staphylococcus intermedius and Staphylococcus hyicus. subsp. hyicus
Isolates from Veterinary Clinical Specimens". Clin.Microbiol:14: 671-673.
14. van Griethuysen, A., Bes, M., Etienne, J., Zbinden, R. and Kluytmans,
J. (2000). "An International Multicenter Evaluation of a new Latex Agglutination
Test for Identification of Staphylococcus aureus". Clin Microbiol. and
Infect. 6 sup 1: 163.
15. Schnitzler, N., Rainer, M., Conrads, G., Frank, D. and Haase, G. (1988). “ Staphylococcus
lugdunensis: Report of a case of Peritonitis and an Easy-To-
Perform Screening Strategy”. J. Clin.Microbiol. 26: 1939–1949.
16. Ann-Herbert, A., Crowder, C. G., Hancock, G. A., Jarvis, W. R. and Thornsberry,
C. (1998). “Characteristics of Coagulase-Negative-Staphylococci
That Help Differentiate These Species of the Family Micrococcaceae”. J.
Clin.Microbiol. 36: 812–813.
17. Gregson, D. B., Low, D. E., Skulnick, M.and Simor, A. E. (1988). “Problems
with Rapid Agglutination Methods for Identification of Staphylococcus aureus
When Staphylococcus saprophyticus Is Being Tested”. J. Clin. Microbiol.
26:1398–1399.
18. Myhre, E. B. and Kuusela, P. (1983).“ Binding of Human Fibronectin
to Group A, C and G Streptococci”.Infect. Immun. 40: 29–34.
19. Runehagen, A., Schonbeck, C., Hedneru, Hessel, B. and Kronvall, G.(1981). “Binding
of Fibrinogen Degradation Products to S. aureus and to ß-Hemolytic
Streptococci Group A, C and G”. Acta. path. microbiol. Scand.,
Sect B. 89: 49–55.