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Diagnostic Reagents

OXOID LISTERIA TEST KIT

CODE: DR1126

The Oxoid Listeria Test Kit is a rapid latex agglutination test for the presumptive identification of Listeria spp. in selective and/or enrichment cultures. The Oxoid Listeria Test Kit will confirm the presence of Listeria spp. in culture and should be used in conjunction with biochemical analysis (e.g. MicobactTM 12L Kit-MB1128A) for full identification of Listeria spp.

Introduction
Listeria monocytogenes is the principal causative agent of human listeriosis, which is clinically characterized by Central Nervous System (CNS) involvement in non pregnant individuals and may be associated with infection of the foetus in pregnant women, leading to abortion, stillbirth and premature delivery 1. Contaminated food including dairy and non dairy products are the primary sources of Listeria monocytogenes transmission in both sporadic and outbreak cases 2,3,4. Because of the importance of Listeria monocytogenes in food borne infections and the widespread distribution of Listeria species in the environment, including the intestinal carriage of the organisms by many animal species 5, the rapid identification of Listeria monocytogenes is vital in both the food industry and diagnostic medicine. However, currently the presumptive identification of Listeria monocytogenes relies on the performance of biochemical tests which require prolonged incubation and are time consuming - up to 7 days 6. The Oxoid Listeria Test Kit can provide presumptive identification of Listeria spp. from culture 24 to 48 hours earlier than conventional techniques.

Principle of the Test
Polyvalent antisera are prepared against purified flagellin proteins from Listeria monocytogenes (antigens A, B and C) and Listeria grayi (antigen E) and are used to coat latex particles. When mixed with a suspension containing Listeria spp. the latex particles rapidly agglutinate to form visible clumps. The Oxoid Listeria Test Kit detects all motile strains of Listeria spp.

Components of the Kit
Listeria Latex Reagent. Latex particles coated with rabbit antiserum against Listeria flagellin antigens, preserved with 0.02% Merthiolate. 1 x 5 ml
Listeria positive control antigen 0.5 ml
0.85% isotonic saline, preserved with 0. 09% sodium azide 5 ml
Disposable reaction cards
Disposable mixing sticks
Material required but not provided.
Pasteur pipettes
Forfull proceedure please see product insert.

Storage
The Oxoid Listeria Test Kit should be stored at 2-8°C. Do not freeze. The kit should not be used after the expiry date printed on the outside of the carton.

References
1. Gellin BG and Broome CV. Listeriosis, JAMA (1989) 216, 1313-1320
2. WHO Working Group. Foodborne listeriosis. Bull WHO (1988) 66, 421 428
3. Brackett RE. Presence and persistence of Listeria monocytogenes in food and water. Food Technol (1988) 42, 162
4. Kerr KG, Dealler SF and Lacey RW. Listeria in cook chill food. Lancet (1988) 2, 37 38
5. Bille J and Doyle MP. Listeria and Erysipelot˜rix (1991) Chapt 32 in Manual of Clinical Microbiology, 5th Edition, Eds Albert Balows, William J Hausler, Kenneth L Herrman, Henry D Isenberg, H Jean Shadomy. American Society for Microbiology.
6. McLaughlin J. The identification of Listeria species. Public Health Laboratory Service. DMRQC Newsletter (1988) 3, 1 3

 
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