Part of Thermo Fisher Scientific
OXOID LISTERIA TEST KIT
CODE: DR1126
The Oxoid Listeria Test Kit is a rapid latex agglutination test for the presumptive identification of Listeria spp. in selective and/or enrichment cultures. The Oxoid Listeria Test Kit will confirm the presence of Listeria spp. in culture and should be used in conjunction with biochemical analysis (e.g. MicobactTM 12L Kit-MB1128A) for full identification of Listeria spp.
Introduction
Listeria monocytogenes is the principal causative agent of human listeriosis,
which is clinically characterized by Central Nervous System (CNS) involvement
in
non pregnant individuals and may be associated with infection of the foetus
in pregnant women, leading to abortion, stillbirth and premature delivery
1. Contaminated food including dairy and non dairy products are the primary
sources of Listeria monocytogenes transmission in both sporadic and outbreak cases 2,3,4.
Because of the importance of Listeria monocytogenes in food borne infections and
the widespread distribution of Listeria species in the environment, including
the intestinal carriage of the organisms by many animal species 5, the rapid
identification of Listeria monocytogenes is vital in both the food industry and
diagnostic medicine. However, currently the presumptive identification of
Listeria monocytogenes relies on the performance of biochemical tests which require
prolonged incubation and are time consuming - up to 7 days 6. The Oxoid Listeria
Test Kit can provide presumptive identification of Listeria spp. from culture
24 to 48 hours earlier than conventional techniques.
Principle of the Test
Polyvalent antisera are prepared against purified flagellin proteins from Listeria
monocytogenes (antigens A, B and C) and Listeria grayi (antigen E)
and are used to coat latex particles. When mixed with a suspension containing
Listeria spp. the latex particles rapidly agglutinate to form visible clumps. The
Oxoid
Listeria Test Kit detects all motile strains of Listeria spp.
Components of the Kit
Listeria Latex Reagent. Latex particles coated with rabbit antiserum against
Listeria flagellin antigens, preserved with 0.02% Merthiolate. 1 x 5 ml
Listeria positive control antigen 0.5 ml
0.85% isotonic saline, preserved with 0. 09% sodium azide 5 ml
Disposable reaction
cards
Disposable mixing sticks
Material required but not provided.
Pasteur pipettes
Forfull proceedure please see product insert.
Storage
The Oxoid Listeria Test Kit should be stored at 2-8°C. Do not freeze. The
kit should not be used after the expiry date printed on the outside of the
carton.
References
1. Gellin BG and Broome CV. Listeriosis, JAMA (1989) 216, 1313-1320
2. WHO Working Group. Foodborne listeriosis. Bull WHO (1988) 66, 421 428
3. Brackett RE. Presence and persistence of Listeria monocytogenes in food
and water. Food Technol (1988) 42, 162
4. Kerr KG, Dealler SF and Lacey RW. Listeria in cook chill food. Lancet (1988)
2, 37 38
5. Bille J and Doyle MP. Listeria and Erysipelot˜rix (1991) Chapt 32 in
Manual of Clinical Microbiology, 5th Edition, Eds Albert Balows, William J
Hausler, Kenneth L Herrman, Henry D Isenberg, H Jean Shadomy. American Society
for Microbiology.
6. McLaughlin J. The identification of Listeria species. Public Health
Laboratory
Service. DMRQC Newsletter (1988) 3, 1 3