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IMAGEN™ ADENOVIRUS

Code: K610011

Introduction
IMAGEN™ Adenovirus

50 tests

Principle of the Test
IMAGEN™ Adenovirus is a qualitative direct immunofluorescence test for the detection of adenovirus in clinical samples such as respiratory secretions (eg nasopharyngeal aspirates) and ophthalmic specimens, or for the detection and confirmation of the presence of adenovirus in cell cultures. The kit contains monoclonal antibody conjugated to fluorescein isothiocyanate (FITC). The conjugated reagent binds specifically to an epitope common to the hexon protein found in all serotypes of human adenovirus. The reagent is used in a one-step direct immunofluorescence technique. Specimens are incubated with the FITC conjugated antibody reagent for 15 minutes, and then excess reagent is washed off with phosphate buffered saline (PBS). The stained areas are mounted and viewed microscopically using epifluorescent illumination. If adenovirus is present, characteristic bright apple-green fluorescence is seen within the cytoplasm and/or nucleus of infected cells, contrasting with the red background staining of uninfected cells.