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Laboratory Preparations/Biological Extracts

LIVER DESICCATED

Code: LP0026

Dehydrated whole liver, specially manufactured for the preparation of infusion media. Liver Desiccated is prepared by the dehydration of fresh ox livers under carefully controlled conditions designed to ensure maximum retention of nutritive properties, and is equivalent to five times its weight of fresh liver.

To prepare a liver infusion medium, add 50 grams of Liver Desiccated to 1 litre of distilled water and allow to infuse (with frequent agitation) for 1 hour at 50°C. Boil the mixture for a few minutes to coagulate protein, strain through 60-mesh stainless steel gauze, add 10 grams of Bacteriological Peptone (LP0037) and 5 grams of sodium chloride (LP0005). Adjust the reaction to pH 7.2, boil, strain through gauze as above, and sterilise by autoclaving at 121°C for 15 minutes.

 
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