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Laboratory Preparations/Biological Extracts

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TRYPTONE

Code: LP0042

Tryptone is a pancreatic digest of casein. It can be used in any formulation where a pancreatic or tryptic digest of casein is specified. Casein is the main protein of milk and is a rich source of amino acid nitrogen. The profile shows a broad spread of peaks throughout the molecular weight range characteristic of a pancreatic digest. This hydrolysate is often mentioned in published works, either as a constituent of culture media for metabolic or growth studies, or for other purposes where high performance and uniformity of composition are of paramount importance. It has a high tryptophan content and is therefore used in media for testing the indole reaction.
Tryptone can detect `flat-sour’ or `sulphide’ spoilage organisms in the canning industry and is also used in sterility testing media.
It is a constituent of media used in fermentation processes to produce antibiotics, extra-cellular protein, interferon and diphtheria toxoid.

Typical analysis

(% w/w)

Total Nitrogen

13.3

Amino Nitrogen

3.7

Sodium chloride

0.4

pH (2% solution)

7.3 ± 0.2

 
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