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Quanti-Cult

Sector: Industrial

OXOID QUANTI-CULTPLUS

The Quanti-CultPLUS® range features convenient ready-to-use preserved micro-organisms for use in quality control procedures. Each delivers a specific range of colony forming units (CFUs) and may be used for:
Growth promotion
Bacteriostasis and fungistasis
Microbial limit tests
Media quality control etc.

Use
This product contains <100 colony forming units of preserved micro-organisms per 0.1ml inoculum for use in quality control procedures1,2,3. Colony forming units (CFUs) are specified under defined procedures and growing conditions and cannot be guaranteed under other conditions. Results may vary with inhibitory or selective media or with the same medium of inferior quality.

Description
Each pack contains 10 sets of 2 vials. One vial contains a film of micro-organisms attached to the inside of the cap of the vial. The microbial film can be seen through the red cap as a black area inside the black `O’ ring of the cap. Rehydrating fluid is provided in the second vial, with the blue cap.

Precautions
Quanti-CultPLUS contains live micro-organisms and should be used only by individuals with microbiological training. Properly disinfect any spills and sterilise used containers by autoclaving before final disposal.

Storage
Both rehydrating fluid and micro-organisms should be stored at a refrigerated temperature (2-8°C) until time of analysis.

Expiration date
The reagents are stable until the expiration date on the label when stored as directed.

Evidence of deterioration
Each RED CAP should contain an intact dried film. Do not use if the film has come out of the cap or shows evidence of hydration.

To open
The foil envelope can be cut open with scissors.

To rehydrate
CAUTION
THE REHYDRATED SUSPENSION MUST BE USED WITHIN 30 MINUTES AFTER THE 15 MINUTE REHYDRATION PERIOD. DO NOT PLAN TO USE A REHYDRATED SAMPLE THROUGHOUT THE WORKING DAY OR THE FOLLOWING DAY. PROLONGED HOLDING WILL ADVERSELY EFFECT THE NUMBER OF VIABLE ORGANISMS.

1 Remove vials from the refrigerator. Place the rehydrating fluid vial (blue cap) in a 35-37°C incubator to warm.
2 Allow the vial (red cap) containing the microbial film (foil envelope) to warm to room temperature.
3 Remove the vial (red cap) containing the microbial film from the envelope.
4 Remove and discard the blue cap from the rehydrating fluid.
5 Remove the red cap containing the micro-organisms and transfer to the rehydrating fluid vial. Tighten cap.
6 Invert this vial, tap to be sure liquid is in contact with the inside of the cap, and place in 35-37°C incubator in an INVERTED position for 15 minutes* to dissolve the preserved micro-organisms. Autoclavable polyester racks may be used to hold the vials in an inverted position. Please note that the foam is an efficient insulating material and will retard warming or cooling of liquids.
7 Grasp bottom of vial, hold firmly in an inverted position, tap cap gently to mix suspension and aid dissolution. Excessive vigorous shaking will produce foam.

*CAUTION
Look at the cap to make certain that all of the micro-organisms are in solution. Undissolved intact black particles can be seen through the plastic cap and /or vial. If this happens, reinvert the vial and place back in the incubator and observe closely every 1 to 2 minutes for complete dissolution. Undissolved microbial film will cause reduced counts but prolonged heating may also result in incorrect counts.

Inoculation and sample analysis
Quanti-CultPLUS is designed to deliver ten 0.1ml inocula each containing ‹100 CFUs.
Quanti-CultPLUS is suitable for quality control of any quantitative microbiological procedure1 such as evaluation of culture media used for the growth promotion and/or bacteriostasis and fungistasis procedures2 and other media3.

Hold the rehydrated suspension at room temperature throughout the 30 minute use period. Re-mix sample between withdrawals. When inoculating or transferring to a liquid matrix, it is advisable to have the receiving liquid pre-warmed to 35-37°C. Dispensing into a cold liquid may interfere with even distribution of the micro-organisms.

References
1.
Clesceri L.S., Greenberg A.E. & Trussel R.R. eds. 1989. Standard Methods for the Examination of Water and Wastewater. 17th ed. A.P.H.A., A.W.W. A. & W.P.C.F.
2. United Sates Pharmacopoeia XXII 1990, Mack Publishing Co.
3. Mehlman I.J. 1984. Appendix I. Culture media. In Bacteriological Analytical Manual. A.O.A.C. Arlington, VA.

Quanti-CultPLUSEach tube contains 10 packs (10 tests per pack).
Each test is designed to deliver ‹100 cfu / 0.1 ml.
Total diluent volume per vial is 1.2 ml.

Note
The organisms used in Quanti-CultPLUS are derived from original ATCC® stock cultures according to the number shown.
Quanti-CultPLUS are manufactured for Oxoid Ltd by Remel Inc., an FDA approved company (FDA Registration 1625984).
Quanti-CultPLUS is a registered trademark REMEL.
ATCC® is a registered trademark of American Type Culture Collection.
The ATCC Licensed Derivative Emblem™, the ATCC Licensed Derivative word mark®, and the ATCC catalogue marks are trademarks of ATCC. Oxoid Limited is licensed to use these trademarks and to sell products derived from ATCC® cultures.
Look for the ATCC Licensed Derivative Emblem™ for products derived from ATCC® cultures.

 
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