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Molecular Microbiology

BAX® SYSTEM SALMONELLA  KIT (96 tests)

Code: QB0608

Salmonella PCR Assay for the BAX® System from DuPont Qualicon

Salmonella

click here for information on BAX® System Q7

Description
Salmonella are well known for causing food poisoning.  They can be present in virtually any type of food.  Food may become contaminated at any stage of the production process, from the farm to the fork.  If Salmonella is found in a food product, it can have serious implications for both the consumer and the manufacturer: Salmonellosis can be a very debilitating, and sometimes fatal infection; managing a Salmonella food scare can cost a company millions of pounds.  For this reason, International guidelines require manufacturers to show due diligence in ensuring that their product is free from Salmonella and often demand absence of the organism when testing samples.

When Salmonella is present in a food or environmental sample, the genetic-based BAX® System uses the power of the polymerase chain reaction (PCR) to rapidly create (amplify) millions of copies of a unique, Salmonella -specific DNA fragment for reliable detection. BAX® system assays simplify the method by combining all the PCR reagents you need into a single tablet, conveniently packaged inside the PCR tubes contained in each kit. Reducing the number of manual manipulations and pipetting steps, the tabletted reagents improve accuracy and consistency; hands-on time is reduced to a minimum and shelf life is increased.

After standard enrichment, samples are lysed to break open the cell walls and release DNA. This lysate is then used to hydrate the PCR tablets. Processing in the automated thermo-cycler takes less than 4 hours for a full rack of 96 tests. Electronic results appear as positive/negative icons on-screen, and the data can be easily stored, shared or printed.

Applications
The BAX® System detects Salmonella in a variety of foods, including meat, poultry, fish and seafood, fruit and vegetable products, dairy products, chocolate/bakery products, animal feeds and pasta. Validation studies have shown that the BAX® system performs as well as traditional culture methods for detecting Salmonella in food, but with a quicker time to result. With sensitivity/specificity rates of 98%, the BAX system for detecting Salmonella has been adopted by the USDA-FSIS and approved by government agencies and certifying bodies around the world.

 Media for Use with BAX® System Salmonella

 Medium

Code 

 Buffered Peptone Water

 CM0509

 Buffered Peptone Water (ISO)

 CM1049

 Novobiocin Supplement (10mg/vial)

 SR0181

 Tryptone Soya Broth

 CM0129

 Milk Powder

 LP0031

 Brain Heart Infusion Broth

 CM0225

 

Further Infomation
Further information on this product, including details of adoptions and approvals, can be found on the DuPont Qualicon website.

 
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