Part of Thermo Fisher Scientific

Thermo Scentific
 
 
 

Material Safety Data Sheet

Organisms

Organisms in the Industrial sector this product works with:

Molecular Microbiology

Sector: Industrial

BAX® LISTERIA MONOCYTOGENES KIT (96 tests)

Code: QB0609C

BAX System PCR Assay for Listeria monocytogenes

Listeria monocytogenes

click here for information on BAX System Q7

Description
Listeria monocytogenes infection is frequently asymptomatic.  However, in certain individuals – the very young, the very old and mothers-to-be for example – it can cause a life-threatening disease known as listeriosis.  L. monocytogenes is typically found in the environment. It can also grow at low temperatures and so can survive refrigeration.  Because of these traits and the serious nature of the disease, many manufacturers test for this organism in their food products. International guidelines have been set up which require manufacturers to show due diligence in ensuring that their product is free from Listeria monocytogenes and often demand absence of the organism when testing samples.

When Listeria monocytogenes is present in food or environmental samples, the genetic-based BAX System uses the power of the polymerase chain reaction (PCR) to rapidly create (amplify) millions of copies of a unique, L. monocytogenes -specific DNA fragment for reliable detection.  BAX System assays simplify the method by combining all the PCR reagents you need into a single tablet, conveniently packaged inside the PCR tubes contained in each kit. Reducing the number of manual manipulations and pipetting steps, the tabletted reagents improve accuracy and consistency; hands-on time is reduced to a minimum and shelf life is increased.

After standard enrichment, samples are lysed to break open the cell walls and release DNA. This lysate is then used to hydrate the PCR tablets. Processing in the automated thermo-cycler takes less than 4 hours for a full rack of 96 tests. Electronic results appear as positive/negative icons on-screen, and the data can be easily stored, shared or printed.

Applications
The BAX system detects L. monocytogenes in a variety of foods, including meats, poultry, fish and seafood, fruit and vegetable products, and dairy products. Validation studies have shown that the BAX System performs as well as traditional culture methods for detecting L. monocytogenes in food, but with a quicker time to result (48 hours). With sensitivity/specificity rates of 98%, the BAX system for detecting
L. monocytogenes has been adopted by the USDA-FSIS and approved by government agencies and certifying bodies around the world.

Media for Using with BAX Listeria monocytogenes

 Medium

 Code 

 BAX Listeria Medium (Listeria genus)

 CM1067

 Half Fraser Broth

 

           Fraser Broth Base

 CM0895

           Half Fraser Selective Supplement

 SR0166

 UVM Broth

 

           Listeria Enrichment Broth Base - UVM formulation

 CM0863

           Listeria Primary Selective Erichment Supplement

 SR0142

 MOPS-BLEB Broth

 

           MOPS-BLEB Broth Base

 CM1071

           Listeria Selective Supplement for MOPS-BLEB

 SR0141

Adoptions
USDA-FSIS MLG 8A.01 -applies to red meat, poultry, eggs and environmental samples

Approvals
• AOAC-RI Performance Tested Method 070202 - applies to a wide variety of food; tested on raw meats, fresh produce/vegetables, processed meats, seafood, dairy cultured/non-cultured, egg and egg products, and fruit juices. This assay has been validated for use with both the original and BAX System Q7 instruments.
• International Official Method: submitted for approval
• Health Canada MFLP 28 - applies to a wide variety of food, including raw meats, processed meats, seafood, fresh produce/vegetables, cultured and non-cultured dairy, egg and egg products, and fruit juices.

AOAC 070202Samples of this test kit model were independently evaluated by the AOAC Research Institute and were found to perform to the producer’s specifications as stated in the test kit’s descriptive insert. The producer certifies this kit conforms in all respects to the specifications originally evaluated by the AOAC Research Institute as detailed in the Performance Tested Certificate number 070202.

BAX System Q7

 

 
©2001 - 2024 Oxoid Limited, All rights reserved.
Copyright, Disclaimer and Privacy Policy | Conditions of Sale | About Us | Cookies
Thermo Fisher Scientific Inc.