Part of Thermo Fisher Scientific
Organisms in the Industrial sector this product works with:
BAX® LISTERIA MONOCYTOGENES KIT (96 tests)
Code: QB0609C
BAX System PCR Assay for Listeria monocytogenes
click here for information on BAX System Q7
Description
Listeria monocytogenes infection is frequently asymptomatic. However, in certain individuals – the very young, the very old and mothers-to-be for example – it can cause a life-threatening disease known as listeriosis. L. monocytogenes is typically found in the environment. It can also grow at low temperatures and so can survive refrigeration. Because of these traits and the serious nature of the disease, many manufacturers test for this organism in their food products. International guidelines have been set up which require manufacturers to show due diligence in ensuring that their product is free from Listeria monocytogenes and often demand absence of the organism when testing samples.
When Listeria monocytogenes is present in food or environmental samples, the genetic-based BAX System uses the power of the polymerase chain reaction (PCR) to rapidly create (amplify) millions of copies of a unique, L. monocytogenes -specific DNA fragment for reliable detection. BAX System assays simplify the method by combining all the PCR reagents you need into a single tablet, conveniently packaged inside the PCR tubes contained in each kit. Reducing the number of manual manipulations and pipetting steps, the tabletted reagents improve accuracy and consistency; hands-on time is reduced to a minimum and shelf life is increased.
After standard enrichment, samples are lysed to break open the cell walls and release DNA. This lysate is then used to hydrate the PCR tablets. Processing in the automated thermo-cycler takes less than 4 hours for a full rack of 96 tests. Electronic results appear as positive/negative icons on-screen, and the data can be easily stored, shared or printed.
Applications
The BAX system detects L. monocytogenes in a variety of foods, including meats, poultry, fish and seafood, fruit and vegetable products, and dairy products. Validation studies have shown that the BAX System performs as well as traditional culture methods for detecting L. monocytogenes in food, but with a quicker time to result (48 hours). With sensitivity/specificity rates of 98%, the BAX system for detecting
L. monocytogenes has been adopted by the USDA-FSIS and approved by government agencies and certifying bodies around the world.
Media for Using with BAX Listeria monocytogenes
Medium | Code |
BAX Listeria Medium (Listeria genus) | CM1067 |
Half Fraser Broth |
|
Fraser Broth Base | CM0895 |
Half Fraser Selective Supplement | SR0166 |
UVM Broth |
|
Listeria Enrichment Broth Base - UVM formulation | CM0863 |
Listeria Primary Selective Erichment Supplement | SR0142 |
MOPS-BLEB Broth |
|
MOPS-BLEB Broth Base | CM1071 |
Listeria Selective Supplement for MOPS-BLEB | SR0141 |
Adoptions
USDA-FSIS MLG 8A.01 -applies to red meat, poultry, eggs and environmental samples
Approvals
• AOAC-RI Performance Tested Method 070202 - applies to a wide variety of food; tested on raw meats, fresh produce/vegetables, processed meats, seafood, dairy cultured/non-cultured, egg and egg products, and fruit juices. This assay has been validated for use with both the original and BAX System Q7 instruments.
• International Official Method: submitted for approval
• Health Canada MFLP 28 - applies to a wide variety of food, including raw meats, processed meats, seafood, fresh produce/vegetables, cultured and non-cultured dairy, egg and egg products, and fruit juices.
Samples of this test kit model were independently evaluated by the AOAC Research Institute and were found to perform to the producer’s specifications as stated in the test kit’s descriptive insert. The producer certifies this kit conforms in all respects to the specifications originally evaluated by the AOAC Research Institute as detailed in the Performance Tested Certificate number 070202.