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Differentiate Staphylococcus from Micrococcus by detecting the oxidase enzyme with Thermo Scientific™ Remel™ Microdase Disk.
In 1956, Kovacs1 described the oxidase method that can be used to differentiate Gram-negative bacilli. Faller and Schleifer2 modified Kovacs’ oxidase reagent by using a tetramethyl-p-phenylenediamine (TMPD) in dimethyl sulfoxide (DMSO). Tarrand and Groschel3 further tested modified Kovacs and confirmed it to be far superior to Kovacs’ oxidase reagent.
| Description | Microdase Discs to differentiate staphylococci from micrococci |
| CE Marker | Yes |
| Detectable Analytes | Oxidase Enzyme |
| Format | Vial |
| Quantity | 25 Disks/Vial |
| Unit Size | Each |
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All micrococci have cytochrome c whereas most staphylococci (with a few exceptions) lack this type of cytochrome. The oxidase reagent confirms the presence of cytochrome c4 by exhibiting color change to blue or purple.
- Ready to use: Reagent-impregnated disk. No further preparation required.
- Easy to read: Visible color change that confirms positive test
Positive test: Blue or purple color development within 2 minutes
Negative test: No color change (white to grey color remains) within 2 minutes.
Each Microdase Disk is impregnated with tetramethyl-p-phenylenediamine (TMPD) in dimethyl sulfoxide (DMSO). The DMSO provides solubility and stability against auto-oxidation and enhances the permeability of TMPD into the bacterial cell. In presence of atmospheric oxygen, the oxidase enzyme reacts with TMPD and cytochrome c in the organism to form the colored compound indophenol.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
- Kovacs, N. 1956. Nature (London). 178:703.
- Faller, A. and K.H. Schleifer. 1981. J. Clin. Microbiol. 13:1031-1035.
- Tarrand, J.J. and D.H.M. Groschel. 1982. J. Clin. Microbiol. 16:772-774.
- Baker, J.S. 1984. J. Clin. Microbiol. 19:875-879.
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2°C to 8°C
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