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PathoDx™ Respiratory Virus Panel

Code: R62400

an immunofluorescent assay designed for the qualitative detection of influenza A and B, adenovirus, parainfluenza 1, 2 and 3 and respiratory syncytial virus

Intended use
The PathoDx™ Respiratory Virus Panel (RVP) kit is a direct immunofluorescence test for the qualitative detection of 7 common respiratory viruses (Respiratory Syncytial Virus (RSV), influenza A, influenza B, parainfluenza viruses 1, 2 and 3, and adenovirus) in prepared patient specimens following growth in cell culture.

Principle of the procedure
The PathoDx Repiratory Virus Panel consists of one screening reagent, containing monoclonal antibodies to each respiratory virus, and seven virus-specific monoclonal antibody reagents. All reagents contain monoclonal antibody labelled with fluorescein. Acetone-fixed cells from positive cell culture or shell vial culture are stained with the RVP Screening Reagent and the Negative Control Reagent. The Screening Reagent contains fluorescein-labelled monoclonal antibodies to RSV, influenza A, influenze B, parainfluenza viruses 1, 2 and 3, and adenovirus. They react specifically with any of the above viral antigens, if present in the cell. The Negative Control Reagent contains fluorescein-labelled murine antibodies, which do not react with the viral agents. Unbound antibody and Evans blue counterstain are washed off with buffered saline, and the slide is mounted with buffered glycerol. Under fluoresence microscopy, the viral antigens detected by monoclonal antibodies present will show a characteristic apple-green fluorescence. Uninfected cells will counterstain red with the Evans blue. To identify which of the seven respiratory viruses is reactive with the Screening Reagent, acetone-fixed cell preparations are stained with each of the seven virus-specific reagents, washed free of unbound antibody, mounted with buffered glycerol, and observed under fluorescence microscopy. The responsible virus(es) will show characteristic apple-green fluorescence, with uninfected cells counterstaining red.

Kit contents (200 tests)
RVP Screening Reagent
RVP Negative Control Reagent
RVP Control Slides
RSV Reagent
Influenza A Reagent
Influenza B Reagent
Parainfluenza 1 Reagent
Parainfluenza 2 Reagent
Parainfluenza 3 Reagent
Adenovirus Reagent
RVP Mounting Fluid
Instructions for use