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Material Safety Data Sheet
Required Products
Items which require this product:
Organisms
Organisms this product works with:
Culture Media Supplements
EGG YOLK EMULSION
Code: SR0047
Description
A stabilised emulsion of egg yolk for use in culture media. It may be added
directly to nutrient media for the identification of Clostridium, Bacillus and Staphylococcus species by their lipase activity.
Technique
Examination of Bacteria for Lecithinase
For demonstration of lecithinase activity (especially in the investigation
of `bitty cream’ conditions) add 0.5 or 1.0ml of the emulsion to 10ml of
Blood Agar Base CM0055 or Nutrient Broth No.2 CM0067 - in both cases to clear
the medium, raise the final salt concentration by the addition of 1% of sodium
chloride. After incubation for up to 5 days at 35°C, lecithinase-producers
render the broth opalescent, whilst, on the solid medium, their colonies are
surrounded by zones of opacity.
Lactose Egg-Yolk Milk Agar1,2 - a
medium for the identification of anaerobes which, in addition to serving as
a half antitoxin-Nagler plate, also
demonstrates lactose fermentation and proteolysis. Egg Yolk Emulsion SR0047
is recommended for use in the preparation of the medium.
References
1 Willis A. T. and Hobbs G. (1959) J. Path. Bact. 77. 511-521.
2 Willis A. T. (1977) Anaerobic Bacteriology 3rd Edn. Butterworths, London.
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