Part of Thermo Fisher Scientific

Thermo Scentific
 
 
 

Material Safety Data Sheet

Further Information

Organisms

Organisms this product works with:

Culture Media Supplements

printer friendly version

SKIRROW SELECTIVE MEDIUM

A selective medium for the isolation of campylobacter species at 42°C.

COLUMBIA BLOOD AGAR BASE

Code: CM0331

Formula

gm/litre

Special peptone

23.0

Starch

1.0

Sodium chloride

5.0

Agar

10.0

pH 7.3 ± 0.2

Directions
Add 39g to 1 litre of distilled water. Boil to dissolve and sterilise by autoclaving at 121°C for 15 minutes.

CAMPYLOBACTER SELECTIVE SUPPLEMENT (SKIRROW)

Code: SR0069

Vial contents (each vial is sufficient for 500ml of medium)

 

Vancomycin

5.0 mg

Trimethoprim

2.5 mg

Polymyxin B

1250 IU


Directions
Reconstitute one vial as directed aseptically add the contents to 500 ml of a nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2 , with 10% defibrinated horse or sheep blood SR0050,SR0051 or 5-7% laked horse blood SR0048. Mix gently and pour into sterile Petri dishes.

Description
Campylobacter Selective Supplement is based on the formulation of Skirrow1. The antibiotic supplement is designed to be used at
42°C for optimum selective effect.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated Medium: Straw coloured, free-flowing powder.
Prepared medium: Opaque red-coloured gel.

Reference
1. Skirrow M. B. (1977) BMJ 2. 9-11.

 
©2001 - 2019 Oxoid Limited, All rights reserved.
Copyright, Disclaimer and Privacy Policy | Conditions of Sale | About Us | Cookies
Thermo Fisher Scientific Inc.