Part of Thermo Fisher Scientific
Material Safety Data Sheet
Further Information
Organisms
Organisms this product works with:
Culture Media Supplements
SKIRROW SELECTIVE MEDIUM
A selective medium for the isolation of campylobacter species at 42°C.
COLUMBIA BLOOD AGAR BASE
Code: CM0331
|
Formula |
gm/litre |
|
Special peptone |
23.0 |
|
Starch |
1.0 |
|
Sodium chloride |
5.0 |
|
Agar |
10.0 |
|
pH 7.3 ± 0.2 |
Directions
Add 39g to 1 litre of distilled water. Boil to dissolve and sterilise
by autoclaving at 121°C for 15 minutes.
CAMPYLOBACTER SELECTIVE SUPPLEMENT (SKIRROW)
Code: SR0069
Vial contents (each vial is sufficient for 500ml of medium) |
|
|
Vancomycin |
5.0 mg |
|
Trimethoprim |
2.5 mg |
|
Polymyxin B |
1250 IU |
Directions
Reconstitute one vial as directed aseptically add the
contents
to 500 ml of a nutrient medium cooled to approximately 50°C prepared from
Columbia Agar or Blood Agar Base No.2 ,
with
10% defibrinated horse or sheep blood SR0050,SR0051 or 5-7% laked horse blood
SR0048.
Mix gently and pour into
sterile Petri dishes.
Description
Campylobacter Selective Supplement is based on the formulation of Skirrow1. The
antibiotic supplement is designed to be used at
42°C for optimum selective effect.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry
date on the label.
Store the prepared medium at 2-8°C.
Appearance
Dehydrated Medium: Straw coloured, free-flowing powder.
Prepared medium: Opaque red-coloured gel.
Reference
1. Skirrow M. B. (1977) BMJ 2. 9-11.
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