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Material Safety Data Sheet

Required Products

Items which require this product:

Optional Products

This item can be used optionally with:

Organisms

Organisms this product works with:

Culture Media Supplements

BRUCELLA SELECTIVE SUPPLEMENT

Code: SR0083

A selective supplement for the isolation of Brucella species.

Vial contents (each vial is sufficient for 500ml of medium)

 

Polymixin B

2,500 IU

Bacitracin

12,500 IU

Cycloheximide

50.0mg

Nalidixic acid

2.5mg

Nystatin

50,000 IU

Vancomycin

10.0mg


Directions

Reconstitute one vial as directed, incubate for 10-15 minutes at 37°C. Mix thoroughly and immediately add the vial contents to 500ml of sterile nutrient medium cooled to approximately 50°C prepared from Brucella Medium Base, Columbia Agar Base or Blood Agar Base No.2 together with 5-10% v/v inactivated horse serum SR0035 and 1-5% w/v of a sterile solution of glucose. Mix well and pour into sterile Petri dishes.

 
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