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Material Safety Data Sheet

Further Information

Organisms

Organisms this product works with:

Culture Media Supplements

BLASER-WANG SELECTIVE MEDIUM

A campylobacter medium which can inhibit the growth of Candida albicans.

COLUMBIA BLOOD AGAR BASE

Code: CM0331

Formula

gm/litre

Special peptone

23.0

Starch

1.0

Sodium chloride

5.0

Agar

10.0

pH 7.3 ± 0.2

Directions
Add 39g to 1 litre of distilled water. Boil to dissolve and sterilise by autoclaving at 121°C for 15 minutes.

CAMPYLOBACTER SELECTIVE SUPPLEMENT (BLASER-WANG)

Code: SR0098

Vial contents (each vial is sufficient for 500ml of medium)

per vial
per litre

Vancomycin

5.0 mg

10.0 mg

Polymyxin B

1,250 IU

2,500 IU

Trimethoprim

2.5 mg

5.0 mg

Amphotericin B

1.0 mg

2.0 mg

Cephalothin

7.5 mg

15.0 mg

Directions
Reconstitue one vial as directed add the contents of one vial to 500ml of sterile nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2, with 10% defibrinated horse/sheep blood or 5-7% laked horse blood SR0050, SR0051 or SR0048. Mix gently and pour into sterile Petri dishes.

Description
Campylobacter Selective Supplement Blaser-Wang is based on the formulation of Skirrow1, but with the addition of amphotericin B and cephalothin2.
The inclusion of amphotericin B inhibits the growth of Candida albicans and cephalothin improves the selectivity of the supplement.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated Medium: Straw coloured, free-flowing powder.
Prepared medium: Opaque red-coloured gel.

References

1. Skirrow M.B. (1977) BMJ 2. 9-11.
2. Blaser M.J., Hardesty H.L., Powers B. and Wang W.L.L. (1980) J. Clin. Micro. 11. 309-313.

 
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