Part of Thermo Fisher Scientific
Material Safety Data Sheet
Further Information
Organisms
Organisms this product works with:
For this Organism
Other products used in the isolation of Campylobacter:
Culture Media Supplements
BLASER-WANG SELECTIVE MEDIUM
A campylobacter medium which can inhibit the growth of Candida albicans.
COLUMBIA BLOOD AGAR BASE
Code: CM0331
|
Formula |
gm/litre |
|
Special peptone |
23.0 |
|
Starch |
1.0 |
|
Sodium chloride |
5.0 |
|
Agar |
10.0 |
|
pH 7.3 ± 0.2 |
Directions
Add 39g to 1 litre of distilled water. Boil to dissolve and sterilise
by autoclaving at 121°C for 15 minutes.
CAMPYLOBACTER SELECTIVE SUPPLEMENT (BLASER-WANG)
Code: SR0098
Vial contents (each vial is sufficient for 500ml of medium) |
per vial
|
per litre |
|
Vancomycin |
5.0 mg |
10.0 mg |
|
Polymyxin B |
1,250 IU |
2,500 IU |
|
Trimethoprim |
2.5 mg |
5.0 mg |
|
Amphotericin B |
1.0 mg |
2.0 mg |
|
Cephalothin |
7.5 mg |
15.0 mg |
Directions
Reconstitue one vial as directed add the contents
of one vial
to 500ml of sterile nutrient medium cooled to approximately 50°C prepared
from Columbia Agar or Blood Agar Base No.2,
with
10% defibrinated horse/sheep blood or 5-7% laked horse blood SR0050, SR0051
or SR0048.
Mix gently and pour into
sterile Petri dishes.
Description
Campylobacter Selective Supplement Blaser-Wang is based on the formulation
of Skirrow1, but with the addition of amphotericin B and cephalothin2.
The inclusion of amphotericin B inhibits the growth of Candida albicans and
cephalothin improves the selectivity of the supplement.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry
date on the label.
Store the prepared medium at 2-8°C.
Appearance
Dehydrated Medium: Straw coloured, free-flowing powder.
Prepared medium: Opaque red-coloured gel.
References
1. Skirrow M.B. (1977) BMJ 2. 9-11.
2. Blaser M.J., Hardesty H.L., Powers B. and Wang W.L.L.
(1980) J.
Clin. Micro. 11. 309-313.
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