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Material Safety Data Sheet

Further Information

Optional Products

This item can be used optionally with:

Organisms

Organisms this product works with:

Culture Media Supplements

LEGIONELLA BCYE GROWTH SUPPLEMENT

Code: SR0110

a growth supplement for the isolation of legionellae

Vial contents

SR0110A
(1 vial per 100ml medium)

SR0110C
(1 vial per 500ml medium)

Supplement
per litre
Buffer/Potassium hydroxide
1.0g
5.0g
10g

Ferric pyrophosphate

0.025g

0.125g
0.25g

L-cysteine HCl

0.04g

0.2g
0.4g

a -Ketoglutarate

0.1g

0.5g
1.0g

Directions to prepare Legionella BCYE Agar
Suspend 2.5g of Legionella CYE Agar Base (CM0655) per 90ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Reconstitute one vial SR0110A per 100ml medium or one vial SR0110C per 500ml medium, as directed. Allow medium to cool to 50°C and aseptically add the vial contents. Mix gently and pour into sterile Petri dishes. The final pH of the medium should be 6.9 ± 0.2.

Alternatively, the medium can be further supplemented with one of the following:

  • with Legionella BMPA-α Selective Supplement (SR0111) to give Legionella BMPA Selective Agar
  • with Legionella GVPC Selective Supplement (SR0152) to give Legionella GVPC Selective Agar
  • with Legionella GVPN Selective Supplement (SR0215) to give Legionella GVPN Selective Agar
  • with Legionella MWY Selective Supplement (SR0118) to give Legionella MWY Selective Agar

To confirm an isolate as Legionella spp., please use Legionella BCYE Agar without Cysteine (CM0655 + SR0175) in conjunction with Legionella CYE Agar (CM0655 +SR0110).

 
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