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Material Safety Data Sheet

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Organisms this product works with:

Culture Media Supplements

CYCLOHEXIMIDE 0.1% SOLUTION

CODE: SR0222

A sterile cycloheximide solution for the addition to a range of Oxoid media for the suppression of fungi.

Description
Cycloheximide 0.1% Solution (SR0222), supplied in a glass bottle with screw-cap, is safer and easier to use than cycloheximide powder. The required volume can be simply aliquoted from the bottle (stored in the dark at 2-8ºC) and added to the medium before or after sterilisation.
Suitable for addition to a range of media, including the following (please refer to The Oxoid Manual or website dev.oxoid.com for details):

CM0041 SABOURAUD DEXTROSE AGAR
An acidic medium for the isolation of dermatophytes, other fungi and yeasts. Cycloheximide, chloramphenicol and various other antimicrobials have been recommended for addition to Sabouraud Dextrose Agar (CM0041). The antimicrobials chosen and levels at which they are used will depend on the group of organisms that is to be inhibited. e.g. Cryptococcus neoformans, Aspergillus fumigatus and Allescheria boydii are sensitive to cycloheximide.

CM0375 BRAIN HEART INFUSION AGAR
A solid medium which contains the highly nutritious infusions recommended for the cultivation of fastidious organisms. For the selective isolation of fungi, cycloheximide at 0.5 µg/ml (0.5 ml of SR0222/litre of medium) and chloramphenicol (SR0078) at 0.05 µg/ml may be added.

CM0309 W. L. NUTRIENT AGAR & CM0501 W. L. NUTRIENT BROTH
Two media for the determination of the microbiological flora in brewing and fermentation. Cycloheximide may be added to the medium at a final concentration of 4 µg/ml (4 ml of SR0222/litre of medium) for the selective isolation of bacterial contaminants in yeast cultures.

CM0617 + SR0099 BACILLUS CEREUS SELECTIVE AGAR (PEMBA)
A selective and diagnostic medium for the isolation and enumeration of Bacillus cereus in food and other samples. It is recommended that, where large numbers of fungi are expected in the sample, cycloheximide is added to the medium at a final concentration of 40 µg/ml (40 ml of SR0222/litre of medium).

CM0651 UNIVERSAL BEER AGAR
A medium developed for the isolation of beer spoilage organisms. The addition of cycloheximide at 1 µg/ml (1 ml of SR0222/litre of medium) to suppress yeast growth gives a medium that is selective for the detection of bacterial contaminants in yeast cultures.

CM0777 RAKA-RAY AGAR
The addition of phenylethanol and cycloheximide forms a selective medium for the isolation of lactic acid bacteria in beer and brewing processes. Selectivity is achieved by the addition of 3 g/litre of 2-phenylethanol to inhibit Gram-negative organisms and 7 µg/ml of cycloheximide (7 ml of SR0222/litre of medium) to inhibit yeasts.

 
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