Organisms this product works with:
Other products used in the isolation of Clostridium difficile :
OXOID C. DIFFICLE TOXIN A STOOL FILTRATION SYSTEM
Code: TD0975A
INTENDED USE
The Oxoid stool filtration system is an alternative preparation technique
for the isolation of C. difficle toxin A from stool samples prior to
testing with the Oxoid C. difficle Toxin A Test (TD0970A). The system is intended
exclusively for for use with the Oxoid C. difficle Toxin A Test and does not
require the use of a centrifuge.
SAMPLE PREPARATION
1. Note: All components and reagents should be allowed to reach room temperature
(18-30° C) for 30 minutes before use.
2. a) Liquid Stools - Pippette 1ml of sample diluent to the sample cup
of the stool filtration system. Using a pipette, add 100m
of stool to the sample diluent in the the sample cup.
b) Solid or Semi-Solid Stools - Pippette 1ml of sample diluent to the
sample cup. Using a loop, add a 5mm diameter bead-like portion of stool into
the sample cup.
3. Mix the sample thoroughly using a pipette or loop, as appropriate,
to form an even suspension.
4. Place the filter body (with cap in place) into the sample cup, and
push downward on the cap so that the filter body stops approximately 2mm above
the level of the liquid.
5. Allow the particulate matter to settle by standing the stool filter
system on a level surface for 1 minute.
6. Apply further pressure to the cap of the filter body, and continue
to push downwards so that the sample passes through the filter into the filter
body.
TEST PROCEDURE
Remove the toxin test unit from the foil puch and place on a level surface.
Remove the cap from the body of the filter unit. Invert the stool filtration
system and apply 6 drops of the stool filtrate to the sample window of the C.
difficle Toxin A Test unit, by squeezing the sides of the nozzle.
INTERPRETATION OF RESULTS
Following application of sample from the stool filtration system to the
test device results should be interpreted as directed in the C. difficle Toxin
A Test product insert.