Part of Thermo Fisher Scientific

Thermo Scentific
 
 
 

Veggietones

VEGETABLE PEPTONE BROTH

Code: VG0101

A meat-free alternative to Tryptone Soya Broth. A highly nutritious general purpose medium for the growth of bacteria and fungi, which is particularly useful for sterility testing.

Formula

gm/litre

Vegetable Peptone

18.0

Dextrose

2.5

Di-potassium hydrogen phosphate

2.5

Yeast Extract

3.0

Sodium chloride

5.0

pH 7.1 ± 0.2

 
* Adjusted as required to meet performance standards

Directions
Suspend 31 grams of Vegetable Peptone Broth in 1 litre of distilled water and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Since the emergence of Bovine Spongiform Encephalopathy (BSE) in the 1980s and subsequent worries about transmissible spongiform encephalopathies (TSEs)in other species there has been a growing concern over the use of meat and animal derived products in microbiology.
Despite a strict policy of sourcing from countries where BSE is not known, and tight regulation and certification of all raw materials, Oxoid have recognised the need for a range of meat-free products for use within the pharmaceutical industry.
This has led to the development of Vegetable Peptone Broth, a meat-free alternative to traditional Tryptone Soya Broth.
A pea peptone has been developed, and incorporated into Vegetable Peptone Broth. This peptone uses pea flour as a base, and fungal enzymes for digestion.
Vegetable Peptone Broth is an excellent nutritional base for the growth of a wide range of organisms, including many fastidious species.
To ensure that it meets the needs of pharmaceutical companies for sterility testing, the performance of Vegetable Peptone Broth is tested according to the specifications for growth of micro-organisms laid down in the British, US, European, and Japanese Pharmacopoeia(1-4), and the NCCLS.
Vegetable Peptone Broth is also tested to ensure that it gives quick and easy filtration through both cellulose acetate and cellulose nitrate filters.
No raw materials of animal origin have been used in this product. Pea flour has been used as a protein base for this medium and fungal enzymes have been used to make the peptone.

Technique
Excellent growth of microorganisms
Formulated to give a good nutritional base with optimal buffering to allow luxuriant growth of fastidious organisms.
Quick and easy filtration
Designed to give a clear straw coloured broth which has good filtration rates on both cellulose acetate and cellulose nitrate filters.
Performance tested
The medium is quality control tested to meet US, British, European and Japanese Pharmacopoeia performance standards,

Typical Growth Curves:

 

Storage conditions and Shelf life
Vegetable Peptone Broth should be stored tightly capped in the original container at 10-30°C. When stored as directed, the medium will remain stable until the stated expiry date.
Prepared medium may be stored for up to two weeks at 2-8°C in the dark.

Quality control
For quality control of the medium the organisms named below can be used:
Staphylococcus aureus ATCC® 6538P *
Clostridium sporogenes ATCC® 19404 *
Clostridium perfringens ATCC® 13124
Candida albicans ATCC® 10231 *
Bacillus subtilis ATCC® 6633 *
Pseudomonas aeruginosa ATCC® 9027 *
Micrococcus luteus ATCC® 9341 *
Escherichia coli ATCC® 8739 *
Aspergillus brasiliensis ATCC® 16404 *
* This organism is available as a Culti-Loop®

Precautions
Vegetable Peptone Broth must only be used for in vitro diagnostic purposes.
Do not use Vegetable Peptone Broth beyond the expiry date or if the product shows any sign of deterioration.

References
1. British Pharmacopoeia, Volume No.2 1993
2. Japanese Pharmacopoeia, 12th Edition, Supplement II
3. European Pharmacopoeia, 3rd Edition 1998
4. United States Pharmacopoeia, 23 NF 18 1995

 
©2001 - 2021 Oxoid Limited, All rights reserved.
Copyright, Disclaimer and Privacy Policy | Conditions of Sale | About Us | Cookies
Thermo Fisher Scientific Inc.