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Veggietones

VEGETABLE PEPTONE PHOSPHATE BROTH

Code: VG0200

A meat-free alternative to Tryptose Phosphate Broth CM0283. Suitable for use as a component of tissue culture media and for the cultivation of fastidious organisms.

Formula

gm/litre

Vegetable Peptone No 1

15.0

Dextrose

2.0

Di-basic sodium phosphate

2.5

Yeast Extract

5.0

Sodium chloride

5.0

pH 7.3 ± 0.2 at 25°C

Directions
Suspend 29.5 g of dehydrated powder in 1 litre of distilled water distribute into final containers. Mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. If the reconstituted medium has been stored prior to use, remove dissolved oxygen by placing the tubes in a boiling water bath for 15 minutes and cool without agitation before inoculation.

Description
Vegetable Peptone Phosphate broth has been designed to be comparable to Tryptose Phosphate Broth, a buffered dextrose broth for the growth of fastidious
microorganisms.
Tryptose Phosphate Broth is also used for tissue culture. Vegetable Peptone Phosphate Broth has been tested using Baby Hamster Kidney 21 (BHK 21) cells
and is suitable for use in place of standard Tryptose Phosphate Broth.
Vegetable Peptone Phosphate Broth is made from raw materials that are free from any animal derived products and are GMO free.

Technique
For details of specific techniques please refer to Tryptose Phosphate Broth CM0283.

Storage conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium at 2-8°C.

Appearance
Dehydrated Medium: Straw coloured free flowing powder
Prepared medium: Clear to straw coloured solution.

Quality control

Positive controls:

Expected results

Streptococcus pyogenes ATCC 19615 * Turbid growth.
Staphylococcus aureus ATCC 25923 * Turbid growth.

Negative control:

Uninoculated medium No change

This organism is available as a Culti-Loop®.

 

 
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