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AFNOR Approved Listeria Testing

Oxoid Novel Enrichment Broth – Listeria (ONE Broth) and Oxoid Chromogenic Listeria Agar (OCLA) have been approved by AFNOR (Association Francaise pour la Normalisation) for the detection and differentiation of Listeria monocytogenes and other Listeria species from food samples in just 48 hours.

ONE Broth allows optimal resuscitation, recovery and growth of even low numbers of Listeria species from a single, 24-hour enrichment incubation. This saves time and materials compared to dual broth methods (such as ISO 11920-1:19971) and allows the enriched sample to be plated directly onto OCLA as soon as growth is detected.

OCLA detects ß-glucosidase activity, common to all Listeria species, and the lecithinase activity of L. monocytogenes and pathogenic L. ivanovii, allowing the detection and differentiation of L. monocytogenes and other Listeria species in only 24 hours.

Together, ONE Broth and OCLA provide presumptive identification of Listeria species in just 48 hours – up to 3 days earlier than traditional methods. The use of just one broth and a single agar plate reduces ‘hands-on’ time, frees up resources and leads to a lower cost per test. ONE Broth and OCLA were validated by AFNOR against ISO 11290-1:1997 and shown to give equivalent results2.

A product datasheet (folio number 1059) for this AFNOR approved method is now available from Oxoid. For this and further information about ONE Broth (product codes CM1066 and SR0234E) and OCLA (product codes CM1080, SR0227E and SR0228E), please telephone Val Kane on +44 (0)1256 841144 or email val.kane@oxoid.com.